Departments of Pharmacology, Charlottesville, VA, USA.
Neuroscience Graduate Program, University of Virginia, Charlottesville, VA, USA.
Nat Commun. 2020 Apr 3;11(1):1679. doi: 10.1038/s41467-020-15408-4.
Aldosterone-producing zona glomerulosa (zG) cells of the adrenal gland arrange in distinct multi-cellular rosettes that provide a structural framework for adrenal cortex morphogenesis and plasticity. Whether this cyto-architecture also plays functional roles in signaling remains unexplored. To determine if structure informs function, we generated mice with zG-specific expression of GCaMP3 and imaged zG cells within their native rosette structure. Here we demonstrate that within the rosette, angiotensin II evokes periodic Ca3-dependent calcium events that form bursts that are stereotypic in form. Our data reveal a critical role for angiotensin II in regulating burst occurrence, and a multifunctional role for the rosette structure in activity-prolongation and coordination. Combined our data define the calcium burst as the fundamental unit of zG layer activity evoked by angiotensin II and highlight a novel role for the rosette as a facilitator of cell communication.
肾上腺球状带产生醛固酮（zG）细胞排列成独特的多细胞玫瑰花结，为肾上腺皮质形态发生和可塑性提供了结构框架。这种细胞结构是否也在信号转导中发挥功能作用尚不清楚。为了确定结构是否决定功能，我们生成了 zG 细胞特异性表达 GCaMP3 的小鼠，并在其天然玫瑰花结结构内对 zG 细胞进行成像。在这里，我们证明在玫瑰花结内，血管紧张素 II 引发周期性的 Ca3 依赖性钙事件，形成爆发，其形式具有典型特征。我们的数据揭示了血管紧张素 II 在调节爆发发生中的关键作用，以及玫瑰花结结构在延长和协调活动中的多功能作用。我们的数据将钙爆发定义为血管紧张素 II 诱导的 zG 层活动的基本单位，并强调了玫瑰花结作为细胞通讯促进者的新作用。
