来自骨骼的含γ-羧基谷氨酸蛋白的特性鉴定。
Characterization of a gamma-carboxyglutamic acid-containing protein from bone.
作者信息
Price P A, Otsuka A A, Poser J W, Kristaponis J, Raman N
出版信息
Proc Natl Acad Sci U S A. 1976 May;73(5):1447-51. doi: 10.1073/pnas.73.5.1447.
Abstract
A gamma-carboxyglutamic acid-containing protein has been purified from the calcified tissues of several vertebrates. The presence of three-gamma-carboxyglutamic acid residues in the bovine protein was established by alkaline hydrolysis and amino acid analysis, a method based upon studies with synthetic gamma-carboxyglutamic acid. The identity of gamma-carboxyglutamic acid in the bovine protein was established by mass spectroscopy on the unknown amino acid isolated from alkaline hydrolysates.
摘要
一种含γ-羧基谷氨酸的蛋白质已从几种脊椎动物的钙化组织中纯化出来。通过碱性水解和氨基酸分析确定了牛蛋白中存在三个γ-羧基谷氨酸残基，该方法基于对合成γ-羧基谷氨酸的研究。通过对从碱性水解产物中分离出的未知氨基酸进行质谱分析，确定了牛蛋白中γ-羧基谷氨酸的身份。
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