哺乳动物染色质的无细胞转录：用哺乳动物RNA聚合酶从骨髓染色质转录珠蛋白信使RNA序列

Cell-free transcription of mammalian chromatin: transcription of globin messenger RNA sequences from bone-marrow chromatin with mammalian RNA polymerase.

作者信息

Steggles A W, Wilson G N, Kantor J A, Picciano D J, Falvey A K, Anderson W F

出版信息

Proc Natl Acad Sci U S A. 1974 Apr;71(4):1219-23. doi: 10.1073/pnas.71.4.1219.

DOI:10.1073/pnas.71.4.1219

PMID:4364529

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388196/

Abstract

A mammalian cell-free transcriptional system was developed in which mammalian RNA polymerase synthesizes globin messenger RNA sequences from bone-marrow chromatin. The messenger RNA sequences are detected by measurement of the ability of the transcribed RNA to hybridize with globin complementary DNA. The globin complementary DNA is synthesized by the enzyme from avian myeloblastosis virus, RNA-directed DNA polymerase, with purified globin messenger RNA as template. The specificity of the globin complementary DNA in annealing reactions was verified by preparing DNA complementary to liver messenger RNA and showing that the globin and liver complementary DNAs are specific for their own messenger RNAs. Both DNA-dependent RNA polymerase II from sheep liver and RNA polymerase from Escherichia coli can transcribe globin messenger RNA sequences from rabbit bone-marrow chromatin; however, the mammalian enzyme appears to be more specific in that globin gene sequences represent a higher proportion of the RNA synthesized. Neither polymerase can transcribe globin messenger RNA sequences from rabbit-liver chromatin. This cell-free assay system should be useful in searching for mammalian transcriptional regulatory factors.

摘要

开发了一种无细胞哺乳动物转录系统，其中哺乳动物RNA聚合酶从骨髓染色质合成珠蛋白信使RNA序列。通过测量转录的RNA与珠蛋白互补DNA杂交的能力来检测信使RNA序列。珠蛋白互补DNA由禽成髓细胞瘤病毒的酶、RNA指导的DNA聚合酶以纯化的珠蛋白信使RNA为模板合成。通过制备与肝脏信使RNA互补的DNA并表明珠蛋白和肝脏互补DNA对其自身的信使RNA具有特异性，验证了珠蛋白互补DNA在退火反应中的特异性。来自绵羊肝脏的依赖DNA的RNA聚合酶II和来自大肠杆菌的RNA聚合酶都可以从兔骨髓染色质转录珠蛋白信使RNA序列；然而，哺乳动物酶似乎更具特异性，因为珠蛋白基因序列在合成的RNA中占更高比例。两种聚合酶都不能从兔肝脏染色质转录珠蛋白信使RNA序列。这种无细胞检测系统在寻找哺乳动物转录调节因子方面应该是有用的。

相似文献

Cell-free transcription of mammalian chromatin: transcription of globin messenger RNA sequences from bone-marrow chromatin with mammalian RNA polymerase.哺乳动物染色质的无细胞转录：用哺乳动物RNA聚合酶从骨髓染色质转录珠蛋白信使RNA序列

Proc Natl Acad Sci U S A. 1974 Apr;71(4):1219-23. doi: 10.1073/pnas.71.4.1219.

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Cell-free transcription of mammalian chromatin. Quantitative measurement of newly synthesized globin messenger RNA sequences.哺乳动物染色质的无细胞转录。新合成的珠蛋白信使RNA序列的定量测定。

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Strand-selective transcription of globin genes in rabbit erythroid cells and chromatin.兔红细胞和染色质中珠蛋白基因的链特异性转录

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本文引用的文献

Proteins of chromatin in template restriction. I. RNA synthesis in vitro.模板限制中染色质的蛋白质。I. 体外RNA合成

Biochim Biophys Acta. 1971 Jan 1;228(1):202-11. doi: 10.1016/0005-2787(71)90560-0.

Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.哺乳动物核糖体的结构与功能。I. 活性肝脏核糖体亚基的分离与特性分析。

J Mol Biol. 1970 Oct 14;53(1):1-19. doi: 10.1016/0022-2836(70)90042-2.

Isolation and partial characterization of reticulocyte factors M1 and M2.网织红细胞因子M1和M2的分离及部分特性分析

J Biol Chem. 1970 Nov 10;245(21):5553-9.

Translation of rabbit haemoglobin messenger RNA by thalassaemic and non-thalassaemic ribosomes.地中海贫血和非地中海贫血核糖体对兔血红蛋白信使核糖核酸的翻译。

Nat New Biol. 1971 Jun 16;231(24):205-8. doi: 10.1038/newbio231205a0.

Cell-free hemoglobin synthesis in beta-thalassemia.β地中海贫血中的无细胞血红蛋白合成

Proc Natl Acad Sci U S A. 1970 Dec;67(4):1854-61. doi: 10.1073/pnas.67.4.1854.

Defect in messenger RNA for human hemoglobin synthesis in beta thalassemia.β地中海贫血中人类血红蛋白合成信使核糖核酸的缺陷。

J Clin Invest. 1971 Dec;50(12):2755-60. doi: 10.1172/JCI106778.

Isolation and translation of hemoglobin messenger RNA from thalassemia, sickle cell anemia, and normal human reticulocytes.从地中海贫血、镰状细胞贫血和正常人网织红细胞中分离和翻译血红蛋白信使核糖核酸。

J Clin Invest. 1971 Nov;50(11):2458-60. doi: 10.1172/JCI106745.

Animal DNA-dependent RNA polymerases. 1. Large-scale solubilization and separation of A and B calf-thymus RNA-polymerase activities.动物依赖DNA的RNA聚合酶。1. 小牛胸腺RNA聚合酶A和B活性的大规模溶解与分离。

Eur J Biochem. 1972 Jul 13;28(2):269-76. doi: 10.1111/j.1432-1033.1972.tb01910.x.

Initiation of globin synthesis. Preparation and use of reticulocyte ribosomes retaining initiation region messenger ribonucleic acid fragments.珠蛋白合成的起始。保留起始区信使核糖核酸片段的网织红细胞核糖体的制备与应用。

J Biol Chem. 1972 Sep 10;247(17):5357-68.

Cell-free hemoglobin synthesis. II. Characteristics of the transfer ribonucleic acid-dependent assay system.无细胞血红蛋白合成。II. 依赖转移核糖核酸的检测系统的特性。

J Biol Chem. 1970 May 10;245(9):2342-9.

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