Amicarelli F, Gasbarri A, Masciocco L, Pompili A, Pacitti C, Carlucci G, Palumbo G, Miranda M
Department of Basic and Applied Biology, University of L'Aquila, Coppito-L'Aquila, Italy.
Cell Mol Biol (Noisy-le-grand). 1999 Nov;45(7):1093-7.
Parkinson's disease is a neurodegenerative disorder which is mainly characterized by degeneration of the dopaminergic cells in the nigro-striatal system. Due to a lowered L-tyrosine 3-monooxygenase activity, L-tyrosine is not sufficiently transformed to L-DOPA. To date the most common therapy is the administration of the dopamine precursor L-DOPA, with severe collateral effects. Therefore, the substitution of the lacking tyrosine hydroxylase with tyrosinase might be a novel therapeutical approach that would generate specifically L-DOPA from L-tyrosine. We present here evidence that stereotaxic injection of liposome-entrapped tyrosinase is able to significatively increase the levels of dopamine in the rat brain. The catecholamines L-DOPA, dopamine, L-epinephrine, L-norepinephrine were extracted by acid treatment from the brains and detected by HPLC.
帕金森病是一种神经退行性疾病，主要特征是黑质 - 纹状体系统中多巴胺能细胞的退化。由于L - 酪氨酸3 - 单加氧酶活性降低，L - 酪氨酸不能充分转化为L - 多巴。迄今为止，最常见的治疗方法是给予多巴胺前体L - 多巴，但会产生严重的副作用。因此，用酪氨酸酶替代缺乏的酪氨酸羟化酶可能是一种新的治疗方法，它可以从L - 酪氨酸特异性地生成L - 多巴。我们在此提供证据表明，立体定向注射脂质体包裹的酪氨酸酶能够显著提高大鼠脑中多巴胺的水平。通过酸处理从脑中提取儿茶酚胺L - 多巴、多巴胺、L - 肾上腺素、L - 去甲肾上腺素，并通过高效液相色谱法进行检测。
