Tenjo T, Toyoda M, Okuda J, Watanabe I, Yamamoto T, Tanaka K, Ohtani M, Nohara T, Kawasaki H, Tanigawa N
Department of General and Gastroenterological Surgery, Osaka Medical College, Osaka, Japan.
Oncology. 2000;58(1):45-51. doi: 10.1159/000012078.
Tumor cell apoptosis, proliferation and p27 expression using an in situ apoptosis detection kit, anti-Ki67 antibody and anti-p27 antibody were investigated in 171 colorectal adenocarcinoma specimens, together with the assessment of mutated p53 expression. No apparent association was observed among p27 expression, mutated p53 accumulation, the Ki67 labeling index and the apoptotic index (AI). In the multivariate analysis using the median values as the cut-off points (46.8% for p27 expression and 0.89% for AI), p27 expression was identified as an independent and significant predictor for overall survival. When the present series of patients were dichotomized by these cut-off points to categorize the cases into 4 subgroups, the patients in the group with low p27 expression and a low AI had the poorest prognosis. The assessment of p27 expression and AI therefore may prove valuable in identifying patients with colorectal adenocarcinoma who may have a poor prognosis.
使用原位凋亡检测试剂盒、抗Ki67抗体和抗p27抗体，在171例大肠腺癌标本中研究肿瘤细胞凋亡、增殖及p27表达，并评估突变型p53表达。未观察到p27表达、突变型p53蓄积、Ki67标记指数和凋亡指数（AI）之间存在明显关联。在以中位数作为分界点的多因素分析中（p27表达为46.8%，AI为0.89%），p27表达被确定为总生存的独立且显著的预测指标。当根据这些分界点将本系列患者分为两组以形成4个亚组时，p27表达低且AI低的组患者预后最差。因此，评估p27表达和AI可能对识别预后不良的大肠腺癌患者有价值。
