Stroka J, Petz M, Joerissen U, Anklam E
European Commission, Institute for Health and Consumer Protection, Ispra, Italy.
Food Addit Contam. 1999 Aug;16(8):331-8. doi: 10.1080/026520399283902.
Various extractants were investigated concerning their suitability for aflatoxin B1 determinations in different matrices including spices, infant formula and animal feed employing an immunoaffinity clean-up procedure. It was shown that the use of aqueous acetonitrile extractants was limited due to the fact that dry sample material can absorb significant amounts of water from the extractant. This can result in recoveries that are too high and therefore in incorrect values for the aflatoxin concentration if aliquots are taken for further analysis. A correction of the results by recovery calculation using spiked blank material is unsuitable, since material from the same group of food (e.g. paprika powder) or feed can vary significantly in the recovery values. Therefore it is recommended that aqueous methanol extractants are used, since no significant interaction with matrix constituents was observed. In addition, aqueous acetone extractants are a useful alternative with some limitations.
针对采用免疫亲和净化程序在包括香料、婴儿配方奶粉和动物饲料等不同基质中测定黄曲霉毒素B1的适用性，研究了各种萃取剂。结果表明，使用乙腈水溶液萃取剂存在局限性，因为干燥的样品材料会从萃取剂中吸收大量水分。如果取 aliquots 进行进一步分析，这可能导致回收率过高，从而使黄曲霉毒素浓度值不正确。使用加标空白材料通过回收率计算对结果进行校正并不合适，因为来自同一类食品（如辣椒粉）或饲料的材料回收率值可能会有很大差异。因此，建议使用甲醇水溶液萃取剂，因为未观察到与基质成分有明显相互作用。此外，丙酮水溶液萃取剂是一种有一定局限性的有用替代品。
