Lewandrowski K U, Gresser J D, Wise D L, White R L, Trantolo D J
Orthopaedic Research Laboratories, Massachusetts General Hospital, Boston 02114, USA.
Biomaterials. 2000 Feb;21(3):293-8. doi: 10.1016/s0142-9612(99)00180-5.
We have investigated an injectable form of a resorbable bone cement based on in situ crosslinking of the unsaturated polyester, poly(propylene glycol-co-fumaric acid) (PPF). This material, filled with calcium gluconate/hydroxyapatite (CG/HA), cures to a hard cement degradable by hydrolysis. The purpose of this study was to evaluate the osteoconductive properties of this injectable cement. The cement was used as an adjunct to fixation with an intramedullary rod in the rat femoral osteotomy model. Ingrowth of new bone into the cement was examined in vivo. Negative and positive controls with rigid and loose internal fixation were included for comparison. Animals were evaluated histologically and histomorphometrically at 4 weeks postoperatively. Results of this study showed osteoblastic activity and new bone formation at the interface between the femoral bone and the cement in the experimental group. However, there was little bone remodeling at the endosteal surface in positive and negative controls. Histologic evaluation of the cement revealed the formation of cavitations, which likely resulted from leaching of the highly soluble calcium gluconate portion of the filler from the cement. These cavitations were sites of ingrowth of vascular and bony tissues. Intimate contact between the bone cement and the endosteal surface of the cortex was found. Quantitative histomorphometric analysis corroborated these observations. Findings of this study demonstrated the osteoconductivity of this type of injectable PPF-based bone cement.
我们研究了一种基于不饱和聚酯聚（丙二醇 - 共 - 富马酸）（PPF）原位交联的可注射型可吸收骨水泥。这种填充葡萄糖酸钙/羟基磷灰石（CG/HA）的材料固化为可通过水解降解的硬骨水泥。本研究的目的是评估这种可注射骨水泥的骨传导性能。在大鼠股骨截骨模型中，该骨水泥被用作髓内棒固定的辅助材料。在体内检查新骨向骨水泥中的生长情况。纳入了采用刚性和松质内固定的阴性和阳性对照进行比较。术后4周对动物进行组织学和组织形态计量学评估。本研究结果显示，实验组股骨与骨水泥界面处有成骨细胞活性和新骨形成。然而，阳性和阴性对照的骨内膜表面几乎没有骨重塑。对骨水泥的组织学评估显示有空洞形成，这可能是由于填充物中高溶解性的葡萄糖酸钙部分从骨水泥中浸出所致。这些空洞是血管和骨组织生长的部位。发现骨水泥与皮质骨内膜表面紧密接触。定量组织形态计量学分析证实了这些观察结果。本研究结果证明了这种可注射的基于PPF的骨水泥具有骨传导性。
