下半身缺血再灌注后中性粒细胞介导的远隔器官损伤依赖于选择素和补体。

Neutrophil mediated remote organ injury after lower torso ischemia and reperfusion is selectin and complement dependent.

作者信息

Kyriakides C, Austen W G, Wang Y, Favuzza J, Moore F D, Hechtman H B

机构信息

Department of Surgery, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts, USA.

出版信息

J Trauma. 2000 Jan;48(1):32-8. doi: 10.1097/00005373-200001000-00006.

DOI:10.1097/00005373-200001000-00006

PMID:10647562

Abstract

BACKGROUND

Lower torso ischemia and reperfusion leads to remote organ leukosequestration and injury. We now examine the intermediary role of selectins and complement in mediating lung and liver injury after hindlimb ischemia.

METHODS

Mice underwent a 2-hour bilateral tourniquet hind-limb ischemia followed by 3 hours of reperfusion.

RESULTS

Neutrophil depletion significantly decreased lung vascular permeability index (PI), measured by the extravasation of 125I-albumin, and liver injury as assessed by serum alanine aminotransferse levels. Lung PI and serum alanine aminotransferse levels were also reduced in mice treated with recombinant soluble P-selectin glycoprotein ligand-immunoglobulin fusion protein. Complement inhibition with soluble complement receptor type 1 decreased lung PI and serum alanine aminotransferse levels. C5-deficient mice exhibited a similar decrease in lung PI and liver injury. Lung and liver injury were restored in C5-deficient mice reconstituted with wild-type serum.

CONCLUSION

Remote organ injury after lower torso reperfusion is selectin and complement dependent.

摘要

背景

下半身缺血再灌注会导致远隔器官白细胞滞留和损伤。我们现在研究选择素和补体在介导后肢缺血后肺和肝损伤中的中间作用。

方法

小鼠接受2小时双侧止血带后肢缺血，随后再灌注3小时。

结果

中性粒细胞耗竭显著降低了通过125I-白蛋白外渗测量的肺血管通透性指数（PI），以及通过血清丙氨酸转氨酶水平评估的肝损伤。用重组可溶性P-选择素糖蛋白配体-免疫球蛋白融合蛋白处理的小鼠的肺PI和血清丙氨酸转氨酶水平也降低。用可溶性补体受体1抑制补体可降低肺PI和血清丙氨酸转氨酶水平。C5缺陷小鼠的肺PI和肝损伤也有类似程度的降低。用野生型血清重建的C5缺陷小鼠的肺和肝损伤得以恢复。