Aungpraphapornchai P, Griffin H G, Gasson M J
Institute of Food Research, Norwich Research Park, Colney, UK.
DNA Seq. 1999;10(3):163-72. doi: 10.3109/10425179909033942.
Diacetyl is produced by strains of lactic acid bacteria used in the dairy industry. Production of this important flavour compound could be increased by genetic manipulation of genes encoding enzymes involved in diacetyl metabolism. This paper reports the cloning and sequencing of the gene (dar) encoding diacetyl-acetoin reductase from Lactococcus lactis. Analysis of the DNA sequence of the dar gene and surrounding area revealed the presence of a putative operon with similarity to the family of ABC transporter systems. The dar gene has been deleted from the chromosome by double cross-over homologous recombination.
双乙酰由乳制品工业中使用的乳酸菌菌株产生。通过对参与双乙酰代谢的酶的编码基因进行基因操作，可以增加这种重要风味化合物的产量。本文报道了来自乳酸乳球菌的双乙酰 - 乙偶姻还原酶编码基因（dar）的克隆和测序。对dar基因及其周边区域的DNA序列分析揭示了一个与ABC转运蛋白系统家族相似的假定操纵子的存在。通过双交换同源重组，dar基因已从染色体上删除。
