乳酸乳球菌中双乙酰代谢途径的基因操作。
Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis.
作者信息
Swindell S R, Benson K H, Griffin H G, Renault P, Ehrlich S D, Gasson M J
机构信息
Institute of Food Research, Colney, United Kingdom.
出版信息
Appl Environ Microbiol. 1996 Jul;62(7):2641-3. doi: 10.1128/aem.62.7.2641-2643.1996.
Abstract
Diacetyl is an important food flavor compound produced by certain strains of citrate-metabolizing lactic acid bacteria. Citrate is converted to pyruvate, from which diacetyl is produced via intermediate alpha-acetolactate. This paper reports the cloning and analysis of the gene (aldB) encoding alpha-acetolactate decarboxylase from Lactococcus lactis MG1363. Deletion of the MG1363 chromosomal aldB gene was achieved by double crossover homologous recombination. The mutant strain was found to produce diacetyl; alpha-acetolactate decarboxylase activity was eliminated. Overexpression of the cloned ilvBN genes (encoding an alpha-acetolactate synthase) in the aldB deletion strain produced even higher levels of alpha-acetolactate, acetoin, and diacetyl.
摘要
双乙酰是由某些能代谢柠檬酸盐的乳酸菌菌株产生的一种重要的食品风味化合物。柠檬酸盐被转化为丙酮酸，双乙酰通过中间产物α-乙酰乳酸由丙酮酸产生。本文报道了来自乳酸乳球菌MG1363的编码α-乙酰乳酸脱羧酶的基因（aldB）的克隆与分析。通过双交换同源重组实现了MG1363染色体aldB基因的缺失。发现突变株能产生双乙酰；α-乙酰乳酸脱羧酶活性被消除。在aldB缺失菌株中克隆的ilvBN基因（编码α-乙酰乳酸合酶）的过表达产生了更高水平的α-乙酰乳酸、乙偶姻和双乙酰。
相似文献
1
Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis.乳酸乳球菌中双乙酰代谢途径的基因操作。
Appl Environ Microbiol. 1996 Jul;62(7):2641-3. doi: 10.1128/aem.62.7.2641-2643.1996.
2
Imbalance of leucine flux in Lactococcus lactis and its use for the isolation of diacetyl-overproducing strains.乳酸乳球菌中亮氨酸通量的失衡及其在筛选双乙酰高产菌株中的应用。
Appl Environ Microbiol. 1996 Jul;62(7):2636-40. doi: 10.1128/aem.62.7.2636-2640.1996.
3
Dual role of alpha-acetolactate decarboxylase in Lactococcus lactis subsp. lactis.α-乙酰乳酸脱羧酶在乳酸乳球菌乳酸亚种中的双重作用。
J Bacteriol. 1997 Oct;179(20):6285-93. doi: 10.1128/jb.179.20.6285-6293.1997.
4
Metabolic engineering of Lactococcus lactis: influence of the overproduction of alpha-acetolactate synthase in strains deficient in lactate dehydrogenase as a function of culture conditions.乳酸乳球菌的代谢工程：乳酸脱氢酶缺陷型菌株中α-乙酰乳酸合酶过量表达对培养条件的影响
Appl Environ Microbiol. 1995 Nov;61(11):3967-71. doi: 10.1128/aem.61.11.3967-3971.1995.
5
Diacetyl and alpha-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in alpha-acetolactate decarboxylase and have a low lactate dehydrogenase activity.乳酸乳球菌乳酸亚种双乙酰变种突变体过量产生双乙酰和α-乙酰乳酸，这些突变体缺乏α-乙酰乳酸脱羧酶且乳酸脱氢酶活性较低。
Appl Environ Microbiol. 2000 Dec;66(12):5518-20. doi: 10.1128/AEM.66.12.5518-5520.2000.
6
Glucose/citrate cometabolism in Lactococcus lactis subsp. lactis biovar diacetylactis with impaired alpha-acetolactate decarboxylase.乳酸乳球菌乳酸亚种双乙酰变种中α-乙酰乳酸脱羧酶受损时的葡萄糖/柠檬酸盐共代谢
Metab Eng. 1999 Oct;1(4):291-8. doi: 10.1006/mben.1999.0123.
7
α-Acetolactate synthase of Lactococcus lactis contributes to pH homeostasis in acid stress conditions.乳酸乳球菌的α-乙酰乳酸合酶在酸胁迫条件下有助于维持pH稳态。
Int J Food Microbiol. 2014 Oct 1;188:99-107. doi: 10.1016/j.ijfoodmicro.2014.07.017. Epub 2014 Jul 27.
8
Efficient production of α-acetolactate by whole cell catalytic transformation of fermentation-derived pyruvate.利用发酵衍生的丙酮酸进行全细胞催化转化生产 α-乙酰乳酸。
Microb Cell Fact. 2019 Dec 29;18(1):217. doi: 10.1186/s12934-019-1271-1.
9
Activation of the diacetyl/acetoin pathway in Lactococcus lactis subsp. lactis bv. diacetylactis CRL264 by acidic growth.乳酸乳球菌乳酸亚种双乙酰乳酸亚种CRL264中双乙酰/乙偶姻途径通过酸性生长被激活。
Appl Environ Microbiol. 2008 Apr;74(7):1988-96. doi: 10.1128/AEM.01851-07. Epub 2008 Feb 1.
10
Mutation of the oxaloacetate decarboxylase gene of Lactococcus lactis subsp. lactis impairs the growth during citrate metabolism.乳酸乳球菌乳亚种草酰乙酸脱羧酶基因的突变会损害柠檬酸盐代谢过程中的生长。
J Appl Microbiol. 2008 Jan;104(1):260-8. doi: 10.1111/j.1365-2672.2007.03582.x. Epub 2007 Oct 9.
引用本文的文献
1
A thermodynamic atlas of carbon redox chemical space.碳氧化还原化学空间的热力学图集。
Proc Natl Acad Sci U S A. 2020 Dec 29;117(52):32910-32918. doi: 10.1073/pnas.2005642117. Epub 2020 Dec 21.
2
Beverage and Food Fragrance Biotechnology, Novel Applications, Sensory and Sensor Techniques: An Overview.饮料和食品香料生物技术、新应用、感官与传感技术：综述
Foods. 2019 Dec 5;8(12):643. doi: 10.3390/foods8120643.
3
Rhizobacterium-derived diacetyl modulates plant immunity in a phosphate-dependent manner.根际细菌衍生的双乙酰以依赖磷酸盐的方式调节植物免疫。
EMBO J. 2020 Jan 15;39(2):e102602. doi: 10.15252/embj.2019102602. Epub 2019 Dec 5.
4
Effect of Respiratory Growth on the Metabolite Production and Stress Robustness of N87 Cultivated in Cheese Whey Permeate Medium.呼吸生长对在奶酪乳清渗透液培养基中培养的N87代谢产物产生及应激抗性的影响。
Front Microbiol. 2019 Apr 24;10:851. doi: 10.3389/fmicb.2019.00851. eCollection 2019.
5
Analytical method validation and monitoring of diacetyl in liquors from Korean market.韩国市场酒类中二乙酰的分析方法验证与监测
Food Sci Biotechnol. 2017 Jul 13;26(4):893-899. doi: 10.1007/s10068-017-0124-0. eCollection 2017.
6
Suppression of microbial metabolic pathways inhibits the generation of the human body odor component diacetyl by Staphylococcus spp.抑制微生物代谢途径可抑制葡萄球菌属产生人体气味成分双乙酰。
PLoS One. 2014 Nov 12;9(11):e111833. doi: 10.1371/journal.pone.0111833. eCollection 2014.
7
Metabolic engineering of lactic acid bacteria for the production of industrially important compounds.用于生产具有工业重要性化合物的乳酸菌代谢工程。
Comput Struct Biotechnol J. 2012 Oct 29;3:e201210003. doi: 10.5936/csbj.201210003. eCollection 2012.
8
Engineering of cofactor regeneration enhances (2S,3S)-2,3-butanediol production from diacetyl.辅因子再生工程增强了由双乙酰生产（2S,3S）-2,3-丁二醇的能力。
Sci Rep. 2013;3:2643. doi: 10.1038/srep02643.
9
Diacetyl and acetoin production from whey permeate using engineered Lactobacillus casei.利用工程化干酪乳杆菌从乳清渗透液中生产双乙酰和乙偶姻。
J Ind Microbiol Biotechnol. 2009 Sep;36(9):1233-7. doi: 10.1007/s10295-009-0617-9. Epub 2009 Jul 16.
10
Development and Use of a Screening Procedure for Production of (alpha)-Acetolactate by Lactococcus lactis subsp. lactis biovar diacetylactis Strains.开发和使用筛选程序生产（α）-乙酰乳酸的乳球菌乳亚种。双乙酰亚种菌株。
Appl Environ Microbiol. 1997 Feb;63(2):793-5. doi: 10.1128/aem.63.2.793-795.1997.
本文引用的文献
1
Effect of Initial Oxygen Concentration on Diacetyl and Acetoin Production by Lactococcus lactis subsp. lactis biovar diacetylactis.初始氧浓度对德氏乳杆菌亚种乳糖发酵二乙酰变种生产双乙酰和乙酰基的影响。
Appl Environ Microbiol. 1993 Jun;59(6):1893-7. doi: 10.1128/aem.59.6.1893-1897.1993.
2
Citrate Fermentation by Lactococcus and Leuconostoc spp.乳球菌属和肠球菌属的柠檬酸发酵
Appl Environ Microbiol. 1991 Dec;57(12):3535-40. doi: 10.1128/aem.57.12.3535-3540.1991.
3
Imbalance of leucine flux in Lactococcus lactis and its use for the isolation of diacetyl-overproducing strains.乳酸乳球菌中亮氨酸通量的失衡及其在筛选双乙酰高产菌株中的应用。
Appl Environ Microbiol. 1996 Jul;62(7):2636-40. doi: 10.1128/aem.62.7.2636-2640.1996.
4
Metabolic operons in Lactococci.乳酸乳球菌中的代谢操纵子。
Dev Biol Stand. 1995;85:431-41.
5
Rapid isolation of genes from bacterial lambda libraries by direct polymerase chain reaction screening.通过直接聚合酶链反应筛选从细菌λ文库中快速分离基因。
FEMS Microbiol Lett. 1993 Aug 15;112(1):49-53. doi: 10.1111/j.1574-6968.1993.tb06422.x.
6
Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis.乳酸乳球菌中的基因失活：支链氨基酸生物合成
J Bacteriol. 1993 Jul;175(14):4383-90. doi: 10.1128/jb.175.14.4383-4390.1993.
7
Purification and properties of the alpha-acetolactate decarboxylase from Lactococcus lactis subsp. lactis NCDO 2118.乳酸乳球菌乳酸亚种NCDO 2118中α-乙酰乳酸脱羧酶的纯化及性质
FEBS Lett. 1994 Aug 29;351(1):95-9. doi: 10.1016/0014-5793(94)00820-5.
8
Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing.原生质体诱导消除后乳酸乳球菌NCDO 712及其他乳酸链球菌的质粒互补体
J Bacteriol. 1983 Apr;154(1):1-9. doi: 10.1128/jb.154.1.1-9.1983.
9
Isolation and characterization of Streptococcus cremoris Wg2-specific promoters.
Appl Environ Microbiol. 1987 Oct;53(10):2452-7. doi: 10.1128/aem.53.10.2452-2457.1987.
10
Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and alpha-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis.乳酸乳球菌乳亚种双乙酰亚种丙酮酸脱氢酶复合体和α-乙酰乳酸合酶的分离、特性鉴定及生理作用
J Bacteriol. 1992 Jul;174(14):4838-41. doi: 10.1128/jb.174.14.4838-4841.1992.
Zotero 插件