乳酸乳球菌乳酸亚种C2中丙酮酸羧化酶基因的克隆、测序及表达
Cloning, sequencing, and expression of the pyruvate carboxylase gene in Lactococcus lactis subsp. lactis C2.
作者信息
Wang H, O'Sullivan D J, Baldwin K A, McKay L L
机构信息
Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota 55108, USA.
出版信息
Appl Environ Microbiol. 2000 Mar;66(3):1223-7. doi: 10.1128/AEM.66.3.1223-1227.2000.
Abstract
A functional pyc gene was isolated from Lactococcus lactis subsp. lactis C2 and was found to complement a Pyc defect in L. lactis KB4. The deduced lactococcal Pyc protein was highly homologous to Pyc sequences of other bacteria. The pyc gene was also detected in Lactococcus lactis subsp. cremoris and L. lactis subsp. lactis bv. diacetylactis strains.
摘要
从乳酸乳球菌乳酸亚种C2中分离出一个功能性的pyc基因，发现它能弥补乳酸乳球菌KB4中的Pyc缺陷。推导的乳球菌Pyc蛋白与其他细菌的Pyc序列高度同源。在乳酸乳球菌乳脂亚种和乳酸乳球菌乳酸亚种双乙酰乳酸菌株中也检测到了pyc基因。
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