趋化因子在正常胰腺和慢性胰腺炎中的差异表达。

Differential expression of chemokines in normal pancreas and in chronic pancreatitis.

作者信息

Saurer L, Reber P, Schaffner T, Büchler M W, Buri C, Kappeler A, Walz A, Friess H, Mueller C

机构信息

Department of Pathology, University of Bern, Bern, Switzerland.

出版信息

Gastroenterology. 2000 Feb;118(2):356-67. doi: 10.1016/s0016-5085(00)70218-6.

DOI:10.1016/s0016-5085(00)70218-6

PMID:10648464

Abstract

BACKGROUND & AIMS: Cellular infiltrates are present already in early stages of chronic pancreatitis. The mechanisms responsible for their recruitment are unknown. Hence, we determined the differential expression of chemokine genes and their cellular sources in normal and affected pancreatic tissues.

METHODS

Pancreatic tissues from 23 patients with chronic pancreatitis and from 4 normal controls were subjected to in situ hybridization for detecting messenger RNA (mRNA) of the chemokine genes interleukin 8, ENA-78, MIG, MCP-1, and I-309.

RESULTS

Normal pancreatic tissues lack cells expressing mRNA for IL-8, ENA-78, MIG, and MCP-1. In contrast, pancreatic lobuli with mild to moderate signs of tissue alterations strongly expressed MCP-1 mRNA in centroacinar ducts, endothelia, fibroblasts, macrophages, T cells, and occasionally in nerves. Interleukin 8 and ENA-78 mRNA is preferentially detected in centroacinar ducts of pancreatic lobuli with more advanced alterations. Variable numbers of pancreas-infiltrating T cells express MIG mRNA. I-309 mRNA, however, is consistently observed in normal acini and in tissue with mild to moderate signs of tissue alterations.

CONCLUSIONS

The observed differential expression of distinct chemokine genes in pancreatic parenchyma and infiltrates from patients with chronic pancreatitis strongly suggests an involvement of distinct chemokines in the initiation and perpetuation of disease.

摘要

背景与目的

细胞浸润在慢性胰腺炎早期即已存在。但其募集机制尚不清楚。因此，我们确定了趋化因子基因在正常和患病胰腺组织中的差异表达及其细胞来源。

方法

对23例慢性胰腺炎患者和4例正常对照者的胰腺组织进行原位杂交，以检测趋化因子基因白细胞介素8、ENA-78、MIG、MCP-1和I-309的信使核糖核酸（mRNA）。

结果

正常胰腺组织缺乏表达IL-8、ENA-78、MIG和MCP-1 mRNA的细胞。相比之下，有轻度至中度组织改变迹象的胰腺小叶在中央腺泡导管、内皮细胞、成纤维细胞、巨噬细胞、T细胞以及偶尔在神经中强烈表达MCP-1 mRNA。白细胞介素8和ENA-78 mRNA在有更严重改变的胰腺小叶中央腺泡导管中优先检测到。不同数量的胰腺浸润T细胞表达MIG mRNA。然而，I-309 mRNA在正常腺泡和有轻度至中度组织改变迹象的组织中持续存在。