Whalen R G, Butler-Browne G S, Gros F
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2018-22. doi: 10.1073/pnas.73.6.2018.
Pulse-labeled cytoplasmic proteins from cultured fetal calf-muscle cells at various stages of development were analyzed by one- and two-dimensional gel electrophoresis. The high resolution of the two-dimensional technique allows the determination of those protein species that begin to be synthesized after cell fusion. In addition, actin has been found to exist in three forms possessing similar biochemical properties and identical molecular weights but having slightly different isoelectric points. Two of the forms are found in prefusion dividing myoblasts and also in cultured kidney cells. The third form is the only one found in fetal muscle tissue and is predominant in cultures of fused muscle cells. Thus, it would seem that actin can exist in several isozymic forms of which one is specific to fused muscle tissue.
对处于不同发育阶段的培养胎牛肌肉细胞中经脉冲标记的细胞质蛋白进行了一维和二维凝胶电泳分析。二维技术的高分辨率能够确定细胞融合后开始合成的那些蛋白质种类。此外，已发现肌动蛋白以三种形式存在，它们具有相似的生化特性和相同的分子量，但等电点略有不同。其中两种形式存在于融合前分裂的成肌细胞以及培养的肾细胞中。第三种形式是在胎儿肌肉组织中发现的唯一形式，并且在融合肌肉细胞培养物中占主导地位。因此，肌动蛋白似乎可以以几种同工酶形式存在，其中一种对融合的肌肉组织具有特异性。
