Prives J M, Paterson B M
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3208-11. doi: 10.1073/pnas.71.8.3208.
Three components of differentiated muscle membrane, the acetylcholine receptor, acetylcholinesterase (EC 3.1.1.7; acetylcholine hydrolase), and adenylate cyclase [EC 4.6.1.1; ATP pyrophosphate-lyase (cyclizing)], appear simultaneously during myogenesis in cultures of embryonic chick muscle, after the main period of rapid cell fusion. However, unlike the cytoplasmic proteins of differentiated muscle, the elaboration of these membrane components is unaltered when fusion is blocked by lowering the calcium concentration in the medium. These results suggest that membrane differentiation and cytoplasmic differentiation are regulated independently during muscle development.
分化的肌细胞膜的三个成分,即乙酰胆碱受体、乙酰胆碱酯酶(EC 3.1.1.7;乙酰胆碱水解酶)和腺苷酸环化酶[EC 4.6.1.1;ATP焦磷酸裂解酶(环化)],在胚胎鸡肌肉培养物的肌生成过程中,于主要的快速细胞融合期之后同时出现。然而,与分化肌肉的细胞质蛋白不同,当通过降低培养基中的钙浓度来阻止融合时,这些膜成分的形成并未改变。这些结果表明,在肌肉发育过程中,膜分化和细胞质分化是独立调节的。