McKeehan W L, Hamilton W G, Ham R G
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2023-7. doi: 10.1073/pnas.73.6.2023.
The trace element selenium is essential for clonal growth of diploid fibroblasts from human fetal lung (WI-38) in media containing small amounts of serum protein. Maximum growth stimulation is obtained when 30 nM neutralized selenious acid is added to a synthetic medium containing 1.5 mg/ml of dialyzed fetal bovine serum protein (equivalent to a 3% serum concentration). Serum appears to be a source of selenium in most culture media, since higher concentrations of serum protein or whole serum mask the selenium requirement of WI-38 cells. Selenium is also required by a Chinese hamster cell line that can be grown in a protein-free synthetic culture medium.
微量元素硒对于在含有少量血清蛋白的培养基中人类胎儿肺二倍体成纤维细胞（WI-38）的克隆生长至关重要。当向含有1.5 mg/ml透析胎牛血清蛋白（相当于3%血清浓度）的合成培养基中添加30 nM中和亚硒酸时，可获得最大生长刺激。在大多数培养基中，血清似乎是硒的来源，因为更高浓度的血清蛋白或全血清会掩盖WI-38细胞对硒的需求。一种可以在无蛋白合成培养基中生长的中国仓鼠细胞系也需要硒。
