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Critical adjustment of cysteine and glutamine concentrations for improved clonal growth of WI-38 cells.

作者信息

Ham R G, Hammond S L, Miller L L

出版信息

In Vitro. 1977 Jan;13(1):1-10. doi: 10.1007/BF02615497.

DOI:10.1007/BF02615497
PMID:856723
Abstract

Clonal growth of WI-38 cells with a plating efficiency of 45% has been achieved in a synthetic nutrient mixture (MCDB 102) supplemented with either whole or dialyzed fetal bovine serum precisely. Deviation by a factor of three in either direction from the optimum concentration (9.0 x 10(-5) M) eliminates essentially all clonal growth. A high concentration of glutamine (2.5 x 10(-3) M) is also needed for optimum clonal growth.

摘要

相似文献

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本文引用的文献

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THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS.人二倍体细胞株的体外寿命有限。
Exp Cell Res. 1965 Mar;37:614-36. doi: 10.1016/0014-4827(65)90211-9.
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Endogenous copper is cytotoxic to a lymphoma in primary culture which requires thiols for growth.内源性铜对原代培养的淋巴瘤具有细胞毒性，该淋巴瘤生长需要硫醇。
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Long-term culture and cloning system for Trypanosoma brucei gambiense bloodstream forms in semi-defined medium in vitro.
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Development of improved media and culture conditions for clonal growth of normal diploid cells.开发用于正常二倍体细胞克隆生长的改良培养基和培养条件。
In Vitro. 1978 Jan;14(1):11-22. doi: 10.1007/BF02618170.
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Clonal growth of chinese hamster cell lines in protein-free media.中国仓鼠细胞系在无蛋白培养基中的克隆生长。
In Vitro. 1977 Sep;13(9):537-47. doi: 10.1007/BF02627849.
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Diabetes mellitus and genetic prediabetes. Decreased replicative capacity of cultured skin fibroblasts.糖尿病和遗传性糖尿病前期。培养的皮肤成纤维细胞复制能力下降。
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Studies on the nutrition and metabolism of animal cells in serum-free media. I. Serum-free monolayer cultures.无血清培养基中动物细胞营养与代谢的研究。I. 无血清单层培养
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An improved nutrient solution for diploid Chinese hamster and human cell lines.一种改良的用于二倍体中国仓鼠和人类细胞系的营养液。
Exp Cell Res. 1963 Feb;29:515-26. doi: 10.1016/s0014-4827(63)80014-2.
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Clonal growth of diploid Chinese hamster cells in a synthetic medium supplemented with purified protein fractions.二倍体中国仓鼠细胞在添加了纯化蛋白组分的合成培养基中的克隆生长。
Exp Cell Res. 1962 Dec;28:489-500. doi: 10.1016/0014-4827(62)90253-7.
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The serial cultivation of human diploid cell strains.人二倍体细胞株的连续培养。
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The adaptation of additional lines of NCTC clone 929(strain L) cells to chemically defined protein-free medium NCTC 109.将NCTC克隆929（L株）细胞的其他细胞系适应化学成分明确的无蛋白培养基NCTC 109。
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Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.哺乳动物细胞的体外克隆生长；有无饲养层时单个海拉细胞形成的集落的生长特性。
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