Birchler M, Neri G, Tarli L, Halin C, Viti F, Neri D
Department of Applied Biosciences, Swiss Federal Institute of Technology, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland.
J Immunol Methods. 1999 Dec 10;231(1-2):239-48. doi: 10.1016/s0022-1759(99)00160-x.
Angiogenesis, the formation of new blood vessels from pre-existing ones, is a characteristic process which underlies many diseases, including cancer, rheumatoid arthritis and blinding ocular disorders. Antibodies capable of selective targeting and occlusion of neovasculature would open diagnostic and therapeutic opportunities. We have recently demonstrated that phage-derived human antibody fragments with high affinity for the extra-domain B (ED-B) of fibronectin, a marker of angiogenesis, selectively localise in new-forming blood vessels upon intravenous injection. Here, we show that infrared fluorescence methodologies nicely complement radioactive techniques for the study of the antibody-mediated targeting of angiogenesis in a variety of animal models. Methods are presented for the construction and use of infrared fluorescence imagers, as well as for the production and characterisation of recombinant antibodies labeled with infrared fluorophores.
血管生成是指从已有的血管形成新血管的过程，是许多疾病（包括癌症、类风湿性关节炎和致盲性眼部疾病）的一个典型过程。能够选择性靶向和阻断新血管的抗体将带来诊断和治疗机会。我们最近证明，对纤连蛋白额外结构域B（ED-B，一种血管生成标志物）具有高亲和力的噬菌体衍生人抗体片段，静脉注射后可选择性地定位于新生血管中。在此，我们表明，红外荧光方法很好地补充了放射性技术，用于在各种动物模型中研究抗体介导的血管生成靶向。本文介绍了红外荧光成像仪的构建和使用方法，以及用红外荧光团标记的重组抗体的生产和表征方法。
