Kim B G, Shin D H, Jeon G S, Seo J H, Kim Y W, Jeon B S, Cho S S
Department of Anatomy, Seoul National University College of Medicine, Yongon-Dong, Chongno-Gu, Seoul, South Korea.
Brain Res. 2000 Feb 7;855(1):162-5. doi: 10.1016/s0006-8993(99)02374-4.
A certain calcium binding protein (CaBP) has been known to exert a neuroprotective effect in various neurodegenerative diseases. Using the 6-OHDA induced rat Parkinsonian model, we examined if calretinin (CR), one of CaBP family, could play the similar role in the Parkinson's disease because CR is profusely localized in dopaminergic neurons of the substantia nigra pars compacta (SNPC) of the rat. Employing immunohistochemical analyses, we found that the survival rate of CR neurons was significantly higher than that of tyrosine hydroxylase (TH) neurons in the SNPC of the Parkinsonian rat. Furthermore double-labeled fluorescent microscopy revealed that almost all surviving TH neurons were also positive to CR. Our data suggest that CR-positive neurons are less vulnerable to 6-OHDA and CR in the dopaminergic neurons may have a protective function for survival of these neurons in the experimentally induced Parkinsonian rat.
已知某种钙结合蛋白（CaBP）在多种神经退行性疾病中发挥神经保护作用。利用6-羟基多巴胺（6-OHDA）诱导的大鼠帕金森病模型，我们研究了钙视网膜蛋白（CR），即CaBP家族的一员，是否能在帕金森病中发挥类似作用，因为CR大量定位于大鼠黑质致密部（SNPC）的多巴胺能神经元中。通过免疫组织化学分析，我们发现帕金森病大鼠SNPC中CR神经元的存活率显著高于酪氨酸羟化酶（TH）神经元。此外，双标记荧光显微镜显示，几乎所有存活的TH神经元对CR也呈阳性。我们的数据表明，CR阳性神经元对6-OHDA的敏感性较低，多巴胺能神经元中的CR可能对实验诱导的帕金森病大鼠中这些神经元的存活具有保护作用。
