Lindsay D S, Upton S J, Owens D S, Morgan U M, Mead J R, Blagburn B L
Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061-0342, USA.
J Eukaryot Microbiol. 2000 Jan-Feb;47(1):91-5. doi: 10.1111/j.1550-7408.2000.tb00016.x.
A new species of Cryptosporidium is described from the feces of domestic cattle, Bos taurus. Oocysts are structurally similar to those of Cryptosporidium muris described from mice but are larger than those of Cryptosporidium parvum. Oocysts of the new species are ellipsoidal, lack sporocysts, and measure 7.4 x 5.5 microm (range, 6.0-8.1 by 5.0-6.5 microm). The length to width ratio is 1.35 (range, 1.07-1.50). The colorless oocyst wall is < 1 microm thick, lacks a micropyle, and possesses a longitudinal suture at one pole. A polar granule is absent, whereas an oocyst residuum is present. Oocysts were passed fully sporulated and are not infectious to outbred, inbred immunocompetent or immunodeficient mice, chickens or goats. Recent molecular analyses of the rDNA 18S and ITS1 regions and heat-shock protein 70 (HSP-70) genes demonstrate this species to be distinct from C. muris infecting rodents. Based on transmission studies and molecular data, we consider the large form of Cryptosporidium infecting the abomasum of cattle to be a new species and have proposed the name Cryptosporidium andersoni n. sp. for this parasite.
本文描述了一种来自家牛（Bos taurus）粪便中的隐孢子虫新物种。其卵囊在结构上与从小鼠体内分离出的鼠隐孢子虫（Cryptosporidium muris）的卵囊相似，但比微小隐孢子虫（Cryptosporidium parvum）的卵囊大。该新物种的卵囊呈椭圆形，无孢子囊，大小为7.4×5.5微米（范围为6.0 - 8.1×5.0 - 6.5微米）。长宽比为1.35（范围为1.07 - 1.50）。无色的卵囊壁厚度小于1微米，无微孔，在一端有一条纵向缝线。无极粒，但有卵囊残体。排出的卵囊已完全孢子化，对远交、近交的免疫健全或免疫缺陷小鼠、鸡或山羊均无感染性。近期对核糖体DNA 18S和ITS1区域以及热休克蛋白70（HSP - 70）基因的分子分析表明，该物种与感染啮齿动物的鼠隐孢子虫不同。基于传播研究和分子数据，我们认为感染牛皱胃的大型隐孢子虫是一个新物种，并为此寄生虫提议命名为安氏隐孢子虫（Cryptosporidium andersoni n. sp.）。
