Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
Unidad de Investigaciones Agropecuarias (UNIDIA), Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
Acta Parasitol. 2023 Sep;68(3):676-682. doi: 10.1007/s11686-023-00697-8. Epub 2023 Aug 2.
Cryptosporidiosis is a zoonotic infectious disease caused by the protozoan parasite Cryptosporidium spp., frequently found in several animal species, including bats. Several Cryptosporidium genotypes have been described in bats worldwide, suggesting that bats are infected by host-specific Cryptosporidium spp. To date, there are no published reports about Cryptosporidium spp. in bats from Colombia. Therefore, this study aimed to determine the presence and molecular diversity of Cryptosporidium spp. in Colombian bats.
A total of 63 gut samples from three bat species served for molecular detection of Cryptosporidium spp. 18S rDNA gene by qPCR. The sequenced amplicons were used in subsequent phylogenetic analyses to identify them as species or genotypes.
Cryptosporidium spp. qPCR detection occurred in 9.5% (6/63) of bat intestines, and four sequences represented two new genotypes, called Cryptosporidium bat genotypes XIX and XX, were identified.
This study describes the detection of two novel Cryptosporidium bat genotypes, in two species of bats from a region of Colombia, requiring further studies to determine the relationhip between Cryptosporidium and bats in Colombia.
隐孢子虫病是一种由原生动物寄生虫隐孢子虫属引起的人畜共患传染病，常存在于包括蝙蝠在内的几种动物物种中。全世界已在蝙蝠中描述了几种隐孢子虫基因型，表明蝙蝠受到宿主特异性隐孢子虫属的感染。迄今为止，尚无关于哥伦比亚蝙蝠中隐孢子虫属的报道。因此，本研究旨在确定哥伦比亚蝙蝠中隐孢子虫属的存在和分子多样性。
通过 qPCR 对来自三种蝙蝠物种的 63 个肠道样本进行了隐孢子虫属 18S rDNA 基因的分子检测。随后的系统发育分析中使用测序的扩增子将其鉴定为种或基因型。
在 9.5%（6/63）的蝙蝠肠道中检测到隐孢子虫属 qPCR，鉴定出了四个代表两种新基因型的序列，分别命名为隐孢子虫蝙蝠基因型 XIX 和 XX。
本研究描述了在哥伦比亚一个地区的两种蝙蝠中检测到两种新型隐孢子虫蝙蝠基因型，需要进一步研究以确定哥伦比亚隐孢子虫和蝙蝠之间的关系。
