彭氏变形杆菌71 O-特异性多糖的结构及交叉反应性彭氏变形杆菌菌株对新提议血清群O64的分类

Structure of the O-specific polysaccharide of Proteus penneri 71 and classification of cross-reactive P. penneri strains to a new proposed serogroup O64.

作者信息

Zych K, Kocharova N A, Kowalczyk M, Toukach F V, Kaminska D, Shashkov A S, Knirel Y A, Sidorczyk Z

机构信息

Department of General Microbiology, Institute of Microbiology and Immunology, University of Lódz, Poland.

出版信息

Eur J Biochem. 2000 Feb;267(3):808-14. doi: 10.1046/j.1432-1327.2000.01059.x.

DOI:10.1046/j.1432-1327.2000.01059.x

PMID:10651818

Abstract

A neutral O-specific polysaccharide (O-antigen) was isolated from the lipopolysaccharide (LPS) of the bacterium Proteus penneri 71. On the basis of sugar analysis and 1H- and 13C-NMR spectroscopic studies, including two-dimensional COSY, 13C,1H heteronuclear COSY and ROESY, the following structure of the trisaccharide repeating unit of the polysaccharide was established: -->3)-beta-D-GlcpNAc-(1-->4)-beta-D-GlcpNAc-(1-->3)-alpha-D-Galp-(1-- > The polysaccharide has the same carbohydrate backbone as the O-specific polysaccharide of P. penneri 19 and both are similar to that of P. penneri 62 studied by us previously. A cross-reactivity of anti-P. penneri 71, 19 and 62 O-antisera with 11 P. penneri strains was revealed and substantiated at the level of the O-antigen structures. These strains could be divided into three subgroups within a new proposed Proteus O64 serogroup containing P. penneri strains only.

摘要

从彭氏变形杆菌71的脂多糖（LPS）中分离出一种中性O-特异性多糖（O-抗原）。基于糖分析以及一维和二维核磁共振光谱研究，包括二维COSY、13C,1H异核COSY和ROESY，确定了该多糖三糖重复单元的结构如下：-->3)-β-D-氨基葡萄糖-(1-->4)-β-D-氨基葡萄糖-(1-->3)-α-D-半乳糖-(1-- > 该多糖与彭氏变形杆菌19的O-特异性多糖具有相同的碳水化合物主链，并且两者都与我们之前研究的彭氏变形杆菌62的碳水化合物主链相似。揭示了抗彭氏变形杆菌71、19和62 O-抗血清与11株彭氏变形杆菌菌株的交叉反应性，并在O-抗原结构水平上得到证实。这些菌株可在仅包含彭氏变形杆菌菌株的新提议的变形杆菌O64血清群内分为三个亚组。