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彭氏变形杆菌20脂多糖的结构和血清学特征以及交叉反应性彭氏变形杆菌菌株10、16、18、20、32和45在变形杆菌O17血清群中的分类

Structural and serological characterization of the lipopolysaccharide from Proteus penneri 20 and classification of the cross-reacting Proteus penneri strains 10, 16, 18, 20, 32 and 45 in Proteus serogroup O17.

作者信息

Sidorczyk Zygmunt, Toukach Filip V, Zych Krystyna, Arbatsky Nikolay P, Drzewiecka Dominika, Ziółkowski Andrzej, Shashkov Alexander S, Knirel Yuriy A

机构信息

Department of General Microbiology, Institute of Microbiology and Immunology, University of Lódź, Banacha 12/16, 90-237 Lódź, Poland.

出版信息

Arch Immunol Ther Exp (Warsz). 2002;50(5):345-50.

PMID:12455869
Abstract

O-specific polysaccharide (O-antigen) of the lipopolysaccharide of Proteus penneri 20 was studied using sugar analysis along with various one- and two-dimensional NMR spectroscopy techniques. The following structure of the polysaccharide was established: [formula: see text] It has the same carbohydrate backbone structure as that described earlier for P. penneri 16, in which the positions of the O-acetyl groups have not been determined. P. penneri 20 O-antiserum showed a strong cross-reactivity with the lipopolysaccharides of P. penneri 10, 16, 18, 32, 45 and P. mirabilis O17. These data enable classifying these strains together with P. penneri 20 in one Proteus serogroup, O17.

摘要

利用糖分析以及各种一维和二维核磁共振波谱技术,对彭氏变形杆菌20脂多糖的O-特异性多糖(O-抗原)进行了研究。确定了该多糖的以下结构:[分子式:见原文] 它具有与先前描述的彭氏变形杆菌16相同的碳水化合物主链结构,其中O-乙酰基的位置尚未确定。彭氏变形杆菌20 O-抗血清与彭氏变形杆菌10、16、18、32、45以及奇异变形杆菌O17的脂多糖表现出强烈的交叉反应性。这些数据使得能够将这些菌株与彭氏变形杆菌20一起归为一个变形杆菌血清群,即O17。

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