彭氏变形杆菌26株O-特异性多糖的结构与交叉反应性，另一种含有2-乙酰氨基-2,6-二脱氧-L-葡萄糖（N-乙酰-L-昆诺糖胺）的中性变形杆菌O抗原

Structure and cross-reactivity of the O-specific polysaccharide of Proteus penneri strain 26, another neutral Proteus O-antigen containing 2-acetamido-2,6-dideoxy-L-glucose (N-acetyl-L-quinovosamine).

作者信息

Shashkov A S, Arbatsky N P, Widmalm G, Knirel Y A, Zych K, Sidorczyk Z

机构信息

Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.

出版信息

Eur J Biochem. 1998 May 1;253(3):730-3. doi: 10.1046/j.1432-1327.1998.2530730.x.

DOI:10.1046/j.1432-1327.1998.2530730.x

PMID:9654072

Abstract

A neutral O-specific polysaccharide obtained from the lipopolysaccharide of Proteus penneri strain 26 was studied using sugar analysis and 1H and 13C NMR spectroscopy, including two-dimensional NMR techniques. The following structure of the trisaccharide repeating unit was established: -->6)-alpha-D-GlcpNAc-(1-->3)-alpha-L-QuipNAc-(1-->3)-alpha-D-Glcp NAc-(1--> where L-QuiNAc is 2-acetamido-2,6-dideoxy-L-glucose (N-acetyl-L-quinovosamine). Cross-reactivity of the Proteus penneri 26 anti-O serum with other strains of P. penneri isolated in Poland and USA and one strain of P. vulgaris is discussed.

摘要

使用糖分析以及 1H 和 13C NMR 光谱（包括二维 NMR 技术）对从彭氏变形杆菌 26 株脂多糖中获得的一种中性 O 特异性多糖进行了研究。确定了三糖重复单元的以下结构：→6)-α-D-葡萄糖胺-(1→3)-α-L-奎诺糖胺-(1→3)-α-D-葡萄糖胺-(1→，其中 L-奎诺糖胺是 2-乙酰氨基-2,6-二脱氧-L-葡萄糖（N-乙酰-L-奎诺糖胺）。讨论了彭氏变形杆菌 26 抗 O 血清与在波兰和美国分离的其他彭氏变形杆菌菌株以及一株普通变形杆菌的交叉反应性。