Collevatti R G, Brondani R V, Grattapaglia D
Departamento de Ecologia, Universidade de Brasília, Brasília 70910-900, D.F. Brasil; Laboratório de Genética de Plantas, Recursos Genéticos e Biotecnologia-EMBRAPA, C.P. 02372, Brasília, D.F. Brasil.
Heredity (Edinb). 1999 Dec;83 ( Pt 6):748-56. doi: 10.1046/j.1365-2540.1999.00638.x.
In this work we report the development and characterization of 10 microsatellite loci for the endangered tree species Caryocar brasiliense. Using genomic library enrichment, the efficiency of SSR marker development was 14.4% from sequencing data to operationally useful loci. Primer sequences for this set of 10 loci are made available together with their estimates of expected heterozygosity, probability of paternity exclusion and probability of identity. Mendelian inheritance and segregation was confirmed for all 10 loci in open-pollinated half-sib families as well as the absolute transferability of these 10 loci to five other species of the same genus. Number of alleles per locus ranged from 10 to 22 with a mean value of 16 and expected heterozygosity varying from 0.84 to 0.94. The combined probability of genetic identity was on the order of 10-17 clearly demonstrating that SSR multilocus genotypes are likely to be unique and capable of readily discriminating individuals of C. brasiliense. The very high combined probability of paternity exclusion (0.99999995) also indicates that these markers will permit detailed parentage studies in natural populations even in situations where both maternity and paternity are unknown. The battery of microsatellite markers developed and characterized in this study opens a new perspective for the generation of fundamental population genetic data for devising sound collection and conservation procedures for C. brasiliense and related species of the genus.
在本研究中，我们报告了濒危树种巴西猪油果（Caryocar brasiliense）的10个微卫星位点的开发及特征分析。利用基因组文库富集技术，从测序数据到可实际应用的位点，SSR标记开发效率为14.4%。这组10个位点的引物序列及其预期杂合度、父权排除概率和个体识别概率一同公布。在自由授粉的半同胞家系中，所有10个位点均证实了孟德尔遗传和分离现象，并且这10个位点可绝对转移至同一属的其他5个物种。每个位点的等位基因数在10至22之间，平均值为16，预期杂合度在0.84至0.94之间。遗传同一性的联合概率约为10-17，清楚地表明SSR多位点基因型可能是独特的，并且能够轻易区分巴西猪油果个体。极高的父权排除联合概率（0.99999995）也表明，即使在母本和父本均未知的情况下，这些标记也将允许对自然种群进行详细的亲子关系研究。本研究中开发并表征的这一系列微卫星标记为生成基础种群遗传数据开辟了新视角，以便为巴西猪油果及该属相关物种设计合理的采集和保护程序。
