使用混合抗脊髓灰质炎病毒单克隆抗体检测脊髓灰质炎疫苗病毒中的突变体。

Detection of mutants in polio vaccine viruses using pooled antipoliovirus monoclonal antibodies.

作者信息

Horie H, Sato-Miyazawa M, Ota Y, Wakabayashi K, Doi T, Yoshizawa K, Doi Y, Hashizume S

机构信息

Japan Poliomyelitis Research Institute, Kumegawa-cho, Tokyo, 189-0003, Japan.

出版信息

Biologicals. 1999 Sep;27(3):217-26. doi: 10.1006/biol.1999.0211.

DOI:10.1006/biol.1999.0211

PMID:10652177

Abstract

We prepared six monoclonal antibodies (mAbs) for type 1 polioviruses, and analysed their neutralizing specificities for use in safety tests in oral poliomyelitis vaccine (OPV) production. Pools of two or more individual mAbs showed high neutralizing activity against high-titre (approximately 10(7) CCID (50)/25 microl) of Sabin type 1 virus. It was demonstrated that the pooled mAbs can be utilized effectively in detection tests of adventitious viruses, which are among the safety tests in OPV production. Moreover, some pooled mAbs were shown to be capable of detecting very small amounts of type 1 virulent viruses and mutants in high-titre Sabin type 1 virus suspensions. Neutralizing antibody titres of these pooled mAbs decreased with increasing numbers of mutants containing neurovirulent activity in high-titre Sabin type 1 viruses which were repeatedly passaged in culture. It is expected that these pooled mAbs will contribute greatly to safety tests for OPV production.

摘要

我们制备了6种针对1型脊髓灰质炎病毒的单克隆抗体（mAb），并分析了它们的中和特异性，以用于口服脊髓灰质炎疫苗（OPV）生产中的安全性测试。两种或更多种单克隆抗体的组合对高滴度（约10(7) CCID(50)/25微升）的萨宾1型病毒显示出高中和活性。结果表明，组合单克隆抗体可有效地用于OPV生产安全性测试中的外源病毒检测试验。此外，一些组合单克隆抗体能够检测高滴度萨宾1型病毒悬液中极少量的1型强毒株和突变体。在培养中反复传代的高滴度萨宾1型病毒中，随着含有神经毒力活性的突变体数量增加，这些组合单克隆抗体的中和抗体滴度降低。预计这些组合单克隆抗体将对OPV生产的安全性测试做出巨大贡献。