Rieder C R, Parsons R B, Fitch N J, Williams A C, Ramsden D B
Department of Clinical Neuroscience, Queen Elizabeth Hospital, University of Birmingham, Edgbaston, UK.
Neurosci Lett. 2000 Jan 14;278(3):177-80. doi: 10.1016/s0304-3940(99)00932-5.
CYP1B1, a new member of human cytochrome P450 family 1, is involved in the xenobiotic detoxification metabolism and possibly activation of numerous procarcinogens and promutagens. Localization of CYP1B1 in human temporal lobe and its induction in astrocytoma cell line (MOG-G-CCM) by 7,12-dimethylbenz(a)anthracene (DMBA) was investigated using antibodies against human CYP1B1. A single band of approximately 58 kDa size in both human temporal lobe and in MOG-G-CCM was detected by Western blot analysis. Treatment of MOG-G-CCM cells with DMBA resulted in approximately 2.8-fold induction of CYP1B1. CYP1B1 immunoreactivity was detected at the blood-brain interface areas of the temporal lobe as evidenced by co-localization with CD34 antigen. These results suggest that this enzyme may be important in brain xenobiotic metabolism acting as an enzymatic barrier.
细胞色素P450 1B1（CYP1B1）是人类细胞色素P450家族1的新成员，参与外源性物质的解毒代谢，并可能激活多种前致癌物和促诱变剂。使用抗人CYP1B1抗体研究了CYP1B1在人类颞叶中的定位及其在星形细胞瘤细胞系（MOG-G-CCM）中被7,12-二甲基苯并(a)蒽（DMBA）诱导的情况。通过蛋白质免疫印迹分析在人类颞叶和MOG-G-CCM中均检测到一条大小约为58 kDa的条带。用DMBA处理MOG-G-CCM细胞导致CYP1B1诱导约2.8倍。如与CD34抗原共定位所证明，在颞叶的血脑界面区域检测到CYP1B1免疫反应性。这些结果表明，这种酶作为一种酶屏障，可能在脑外源性物质代谢中起重要作用。
