Abd El-Kaream Samir Ali
Lecturer Applied Medical Chemistry, Medical Research Institute, Alexandria University, Egypt.
Biochem Biophys Rep. 2019 Apr 15;18:100637. doi: 10.1016/j.bbrep.2019.100637. eCollection 2019 Jul.
the present study the was done to evaluate chemopreventive and chemotherapeutic anti-tumor potential of some Egyptian plant extract (moringa, graviola, ginger garden cress and artemisinin) against 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinogenesis in Swiss albino mice. chemopreventive and chemotherapeutic evaluation was assessed by monitoring the tumor incidence and tumor volume as well as by analyzing the status of (a) biochemical markers ( and gene expressions), oxidative stress related profile including; total antioxidant capacity (TAC), glutathione reductase (GR) activity, glutathione-s-transferase (GST) activity assay, superoxide dismutase (SOD) activity, catalase (CAT) activity and lipid peroxidation (MDA), renal and hepatic toxicity markers (urea, creatinine, alanine transaminase (alt) activity, aspartate aminotransferase (ast) activity, alkaline phosphatase (ALP) Activity and γ-Glutamyltransferase (GGT) activity also study of (b) biophysical markers (trace and heavy metals (lead (Pb), cadmium (Cd), chromium (Cr), nickel (Ni), iron (Fe), selenium (Se), copper (Cu) and zinc (Zn)), dielectric properties and body water distribution) finally (c) histopathological examination oral administration of increasing dose of moringa, graviola, ginger garden cress and artemisinin extracts, respectively significantly prevented the tumor incidence and tumor volume as well as brought back the status of the above mentioned biochemical and biophysical variables. Histopathological changes also confirmed the formation of tumor tubules and neovascularization after the treatment. Overall, these results suggest that treatment with moringa, graviola, ginger garden cress and artemisinin extracts provided antioxidant defense with strong chemopreventive and chemotherapeutic activity against DMBA-induced mammary tumors.
本研究旨在评估一些埃及植物提取物（辣木、番荔枝、生姜、独行菜和青蒿素）对7,12-二甲基苯并(a)蒽（DMBA）诱导的瑞士白化小鼠乳腺癌变的化学预防和化学治疗抗肿瘤潜力。通过监测肿瘤发生率和肿瘤体积，以及分析（a）生化标志物（以及基因表达）、氧化应激相关指标，包括总抗氧化能力（TAC）、谷胱甘肽还原酶（GR）活性、谷胱甘肽 - S - 转移酶（GST）活性测定、超氧化物歧化酶（SOD）活性、过氧化氢酶（CAT）活性和脂质过氧化（MDA），肾脏和肝脏毒性标志物（尿素、肌酐、丙氨酸转氨酶（ALT）活性、天冬氨酸转氨酶（AST）活性、碱性磷酸酶（ALP）活性和γ-谷氨酰转移酶（GGT）活性）来评估化学预防和化学治疗效果；还研究了（b）生物物理标志物（痕量和重金属（铅（Pb）、镉（Cd）、铬（Cr）、镍（Ni）、铁（Fe）、硒（Se）、铜（Cu）和锌（Zn））、介电特性和身体水分分布）；最后（c）组织病理学检查。分别口服递增剂量的辣木、番荔枝、生姜、独行菜和青蒿素提取物，显著降低了肿瘤发生率和肿瘤体积，并使上述生化和生物物理变量恢复到正常状态。组织病理学变化也证实了治疗后肿瘤小管的形成和新血管生成。总体而言，这些结果表明，辣木、番荔枝、生姜、独行菜和青蒿素提取物的治疗提供了抗氧化防御，对DMBA诱导的乳腺肿瘤具有强大的化学预防和化学治疗活性。
