细胞色素P450 1B1信使核糖核酸在人类中枢神经系统中的表达
Cytochrome P450 1B1 mRNA in the human central nervous system.
作者信息
Rieder C R, Ramsden D B, Williams A C
机构信息
Department of Neurology, University of Birmingham, Queen Elizabeth Hospital, Edgbaston, UK.
出版信息
Mol Pathol. 1998 Jun;51(3):138-42. doi: 10.1136/mp.51.3.138.
AIMS
To study the expression of CYP1B1 in a variety of human and rat cell lines as a means of identifying a new tool for the investigation of gene regulation. In addition, to identify the expression of cytochrome P450 1B1 (CYP1B1) in different regions of the central nervous system (CNS).
METHODS
Reverse transcription-polymerase chain reaction followed by cloning and sequencing were used to detect the expression of CYP1B1 in human cell lines. Poly A+ mRNA from the human spinal cord and from different brain regions was analysed using a CYP1B1 probe labelled with 32PdCTP.
RESULTS
Expression of CYP1B1 was shown in a human astrocytoma cell line (MOG-G-CCM). CYP1B1 mRNA was expressed in a variety of regions of the CNS but with a distinct regional specificity. Expression was highest in the putamen.
CONCLUSIONS
The expression of CYP1B1 in a human astrocytoma enables this cell line to be used in further studies of regulation and function of this gene. The demonstration that CYP1B1 mRNA is expressed in a variety of regions of the CNS suggests a role for this gene in brain and spinal cord metabolism. The regional specificity of expression might explain the focal damage of certain human neurodegenerative diseases.
目的
研究CYP1B1在多种人类和大鼠细胞系中的表达情况，以此作为鉴定一种用于基因调控研究的新工具的手段。此外，确定细胞色素P450 1B1（CYP1B1）在中枢神经系统（CNS）不同区域的表达情况。
方法
采用逆转录-聚合酶链反应，随后进行克隆和测序，以检测CYP1B1在人类细胞系中的表达。使用用32PdCTP标记的CYP1B1探针分析来自人类脊髓和不同脑区的聚腺苷酸加尾mRNA（Poly A+ mRNA）。
结果
在一种人类星形细胞瘤细胞系（MOG-G-CCM）中显示出CYP1B1的表达。CYP1B1 mRNA在CNS的多种区域表达，但具有明显的区域特异性。壳核中的表达最高。
结论
CYP1B1在人类星形细胞瘤中的表达使得该细胞系可用于该基因调控和功能的进一步研究。CYP1B1 mRNA在CNS多种区域表达的证明表明该基因在脑和脊髓代谢中发挥作用。表达的区域特异性可能解释某些人类神经退行性疾病的局灶性损伤。
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