解偶联蛋白——一种有益的能量消散器。

Uncoupling protein--a useful energy dissipator.

作者信息

Klingenberg M

机构信息

Institute of Physical Biochemistry, University of Munich, Germany.

出版信息

J Bioenerg Biomembr. 1999 Oct;31(5):419-30. doi: 10.1023/a:1005440221914.

DOI:10.1023/a:1005440221914

PMID:10653471

Abstract

The structure/function relationship in the uncoupling proteins (UCP) is reviewed, stressing UCP from brown adipose tissue (UCP1) since, so far, nearly no biochemistry is known for the UCP variants UCP2, UCP3, and UCP4. The transport for H+ and Cl- and its dependence on fatty acids in reconstituted vesicles is described. The inhibition and binding of nucleotides to UCP1, in particular, the pH dependence and two-stage binding are analyzed. A model for the role of fatty acid in H+ transport is shown. The role of specific residues in UCP1 is analyzed by directed mutagenesis in a yeast expression system. The different regulation by the cellular energy potential of UCP1 versus UCP3 is discussed.

摘要

本文综述了解偶联蛋白（UCP）的结构/功能关系，重点介绍了棕色脂肪组织中的UCP（UCP1），因为到目前为止，对于UCP变体UCP2、UCP3和UCP4几乎没有已知的生物化学信息。描述了重组囊泡中H⁺和Cl⁻的转运及其对脂肪酸的依赖性。分析了核苷酸对UCP1的抑制和结合作用，特别是pH依赖性和两阶段结合。展示了脂肪酸在H⁺转运中的作用模型。通过酵母表达系统中的定向诱变分析了UCP1中特定残基的作用。讨论了UCP1和UCP3在细胞能量状态下的不同调节方式。