外源性tRNA对32P诱导的可移植骨肉瘤生长的影响。
Influence of exogenous tRNA on growth of transplantable 32P-induced osteosarcomata.
作者信息
Geddes-Dwyer V, Cameron D A
出版信息
Br J Cancer. 1976 Jun;33(6):600-5. doi: 10.1038/bjc.1976.97.
The weights of osteosarcomata that arose 22 days after the s.c. injection of cell suspensions (of whold tumours) that had been exposed in vitro to tRNA were significantly different from the weights of those arising from untreated cells. The tRNA was isolated by phenol extraction and DEAE-cellulose chromatography from eviscerated full-term rat embryos, from rat mesenchymal (granulation) tissue and from rat anaplastic osteosarcomata. Tumours developing from osteosarcoma cells treated with either embryonic or normal mesenchymal tRNA were reduced in weight by 76% and 60% respectively. These effects could not be explained as a toxic consequence, because tumour weight was increased by 128% after exposure of cells to osteosarcoma tRNA. In this osteosarcoma model it appears that tumour weights can be influenced in different ways by tRNA from different sources.
皮下注射经体外暴露于tRNA的细胞悬液（来自整个肿瘤）22天后出现的骨肉瘤重量，与未经处理的细胞产生的骨肉瘤重量显著不同。tRNA通过苯酚提取和DEAE-纤维素色谱法从摘除内脏的足月大鼠胚胎、大鼠间充质（肉芽）组织和大鼠间变性骨肉瘤中分离得到。用胚胎间充质tRNA或正常间充质tRNA处理的骨肉瘤细胞产生的肿瘤重量分别减少了76%和60%。这些作用不能解释为毒性后果，因为细胞暴露于骨肉瘤tRNA后肿瘤重量增加了128%。在这个骨肉瘤模型中，不同来源的tRNA似乎可以以不同方式影响肿瘤重量。
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