Soloviev M M, Ciruela F, Chan W Y, McIlhinney R A
Medical Research Council Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, UK.
J Mol Biol. 2000 Feb 4;295(5):1185-200. doi: 10.1006/jmbi.1999.3436.
Homer proteins bind specifically to the C termini of the metabotropic glutamate receptor mGluR1alpha/a and mGluR5, play a role in their targeting and modulate their synaptic properties. We have discovered that extensive alternative splicing generates a family of 17 Homer proteins. These fall into two distinct groups of 12 "long" Homers, which all have a coiled-coil domain at their C termini, and five "short" Homers, which lack such a domain. All Homers contain the N-terminal sequence responsible for their binding to mGluR1alpha/a receptors and can be co-localised with the recombinantly expressed mGluR1alpha/a protein in HEK-293 cells. The existence of the long and the short variants of each of the Homer-1, Homer-2 and Homer-3 proteins reflects the fundamental principles of Homer functions.
荷马蛋白特异性结合代谢型谷氨酸受体mGluR1α/a和mGluR5的C末端，在其靶向定位中发挥作用并调节其突触特性。我们发现广泛的可变剪接产生了一个由17种荷马蛋白组成的家族。这些蛋白分为两组，一组是12种“长”荷马蛋白，它们在C末端都有一个卷曲螺旋结构域；另一组是5种“短”荷马蛋白，它们缺乏这样的结构域。所有荷马蛋白都含有负责与mGluR1α/a受体结合的N末端序列，并且可以在HEK-293细胞中与重组表达的mGluR1α/a蛋白共定位。荷马-1、荷马-2和荷马-3蛋白各自的长变体和短变体的存在反映了荷马蛋白功能的基本原理。
