Tonolla M, Demarta A, Peduzzi S, Hahn D, Peduzzi R
Cantonal Institute of Bacteriology, Microbial Ecology, University of Geneva, CH-6904 Lugano, Switzerland.
Appl Environ Microbiol. 2000 Feb;66(2):820-4. doi: 10.1128/AEM.66.2.820-824.2000.
Comparative sequence analysis of a 16S rRNA gene clone library from the chemocline of the meromictic Lake Cadagno (Switzerland) retrieved two clusters of sequences resembling sulfate-reducing bacteria within the family Desulfovibrionaceae. In situ hybridization showed that, similar to sulfate-reducing bacteria of the family Desulfobacteriaceae, bacteria of one cluster with similarity values to the closest cultured relatives of between 92.6 and 93.1% resembled free cells or cells loosely attached to other cells or debris. Bacteria of the second cluster closely related to Desulfocapsa thiozymogenes DSM7269 with similarity values between 97. 9 and 98.4% were generally associated with aggregates of different small-celled phototrophic sulfur bacteria, suggesting a potential interaction between the two groups of bacteria.
对瑞士卡达尼奥半咸水湖化学分层带的16S rRNA基因克隆文库进行的比较序列分析,在脱硫弧菌科中检索到了两个类似于硫酸盐还原菌的序列簇。原位杂交显示,与脱硫杆菌科的硫酸盐还原菌类似,其中一个序列簇的细菌与最接近的培养亲缘种的相似性值在92.6%至93.1%之间,类似于游离细胞或松散附着于其他细胞或碎片的细胞。与硫生脱硫荚菌DSM7269密切相关的第二个序列簇的细菌,相似性值在97.9%至98.4%之间,通常与不同的小细胞光合硫细菌聚集体相关,表明这两组细菌之间可能存在相互作用。