Csanády L
Laboratory of Cardiac/Membrane Physiology, The Rockefeller University, 1230 York Avenue, Box 297, New York, New York 10021, USA.
Biophys J. 2000 Feb;78(2):785-99. doi: 10.1016/S0006-3495(00)76636-7.
A method is presented for rapidly extracting single-channel transition rate constants from patch-clamp recordings containing signals from several channels. The procedure is based on a simultaneous fit of the observed dwell-time distributions for all conductance levels, using a maximum likelihood approach. This algorithm allows estimation of single-channel rate constants in cases where more advanced methods may be impractical because of their extremely long computational time. A correction is included for the limited time resolution of the recording system, according to theory developed by Roux and Sauvé (Biophys. J. 48:149-158, 1985), by accounting for the impact of undetected transitions on the dwell-time distributions, and by introducing an improved practical implementation of a fixed dead time for the case of more than one channel. This feature allows application of the method to noisy data, after filtering. A computer program implementing the method is tested successfully on a variety of simulated multichannel current traces.
本文提出了一种从包含多个通道信号的膜片钳记录中快速提取单通道转换速率常数的方法。该程序基于使用最大似然法对所有电导水平下观察到的驻留时间分布进行同时拟合。在更先进的方法由于计算时间极长而不切实际的情况下，此算法允许估计单通道速率常数。根据Roux和Sauvé（《生物物理学杂志》48:149 - 158, 1985）提出的理论，通过考虑未检测到的转换对驻留时间分布的影响，并针对多个通道的情况引入固定死时间的改进实际实现方式，对记录系统有限的时间分辨率进行了校正。此特性允许在滤波后将该方法应用于噪声数据。实现该方法的计算机程序已在各种模拟多通道电流迹线上成功测试。
