血管紧张素原和血管紧张素转换酶多态性与原发性高血压的关系
[The relationship of polymorphism of angiotensinogen and angiotensin converting enzyme with essential hypertension].
作者信息
Ye Q, Wu K, Xie L, Zeng K, Lin J, Lin C
机构信息
Institute of Hypertension Research, The First Affiliated Hospital of Fujian Medical University,Fuzhou, Fujian, 350005 P.R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2000 Feb;17(1):28-31.
OBJECTIVE
In order to investigate the candidate genes for essential hypertension(EH), the authors determined the frequencies of M235T allele variation in exon 2 of angiotensinogen(AGT) gene and angiotensin converting enzyme (ACE) gene in 95 normotensives and 87 hypertensives in Chinese.
METHODS
Polymerase chain reaction combined with restriction enzyme digestion was used to detect the variation AGT gene while PCR was used to determine ACE gene polymorphism.
RESULTS
T235 allele frequency was significantly higher in hypertensive patients than in controls (0.45 vs 0.33, P<0. 05) and it was noticeably higher in male hypertensives. The frequency of ACE D allele in the EH patients with family history was higher than that in controls(0.59 vs 0.41,P<0.05).
CONCLUSION
The variation of AGT gene was involved in the pathogenesis of hypertension, especially in male. The ACE D allele was associated with EH with family history.
目的
为了研究原发性高血压（EH）的候选基因，作者测定了95名血压正常者和87名高血压患者血管紧张素原（AGT）基因第2外显子M235T等位基因变异以及血管紧张素转换酶（ACE）基因的频率。
方法
采用聚合酶链反应结合限制性酶切法检测AGT基因变异，用聚合酶链反应法测定ACE基因多态性。
结果
高血压患者中T235等位基因频率显著高于对照组（0.45对0.33，P<0.05），且在男性高血压患者中尤为明显。有家族史的EH患者中ACE D等位基因频率高于对照组（0.59对0.41，P<0.05）。
结论
AGT基因变异参与了高血压的发病机制，尤其是在男性中。ACE D等位基因与有家族史的EH相关。
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