Mankertz A, Domingo M, Folch J M, LeCann P, Jestin A, Segalés J, Chmielewicz B, Plana-Durán J, Soike D
Robert Koch Institut, P24 (Xenotransplantation), Nordufer 20, 13353, Berlin, Germany.
Virus Res. 2000 Jan;66(1):65-77. doi: 10.1016/s0168-1702(99)00122-7.
The new isolated circovirus variant PCV-2 is discussed to be the etiological agent of a new emerging swine disease with a variable morbidity and high lethality, postweaning multisystemic wasting syndrome (PMWS). PMWS has been diagnosed in North America and West Europe. Clinical signs include dyspnea, loss of weight, lymph node enlargement and lymphocyte depletion in lymphoid tissues. This report describes the characterisation of PCV-2 isolates from animals affected with PMWS from Germany, Spain and France. We could demonstrate the presence of circovirus by electron microscope, in situ hybridisation and PCR. PCR revealed incidence of PCV-2 in many tissues of one infected animal with the exception of heart and nervous system. The phylogenetic analysis of all PCV-2 isolates yet published in the database, showed relationship of isolates from Spain, Germany and France, with three sequences from Canada determined recently and two isolates from Taiwan, while other North American sequences display a separate cluster. PCR screening of randomly collected organ samples from German pigs not affected with PMWS, revealed a rate of infection with PCV-1 of 5% and with PCV-2 of 26.8%, while blood samples showed a lower incidence.
新分离出的圆环病毒变异株PCV - 2被认为是一种新出现的猪病的病原体，这种疾病发病率不定且致死率高，即断奶后多系统消耗综合征（PMWS）。PMWS已在北美和西欧被诊断出来。临床症状包括呼吸困难、体重减轻、淋巴结肿大以及淋巴组织中的淋巴细胞耗竭。本报告描述了从德国、西班牙和法国患有PMWS的动物中分离出的PCV - 2毒株的特征。我们可以通过电子显微镜、原位杂交和PCR证明圆环病毒的存在。PCR显示，在一只受感染动物的许多组织中都有PCV - 2存在，但心脏和神经系统除外。对数据库中已发表的所有PCV - 2毒株进行系统发育分析表明，来自西班牙、德国和法国的毒株与最近确定的来自加拿大的三个序列以及来自台湾的两个分离株存在亲缘关系，而其他北美序列则形成一个单独的簇。对随机收集的未患PMWS的德国猪的器官样本进行PCR筛查发现，PCV - 1的感染率为5%，PCV - 2的感染率为26.8%，而血液样本的发病率较低。
