State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, 130062, China; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China.
Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China.
Virol Sin. 2023 Oct;38(5):663-670. doi: 10.1016/j.virs.2023.08.008. Epub 2023 Sep 1.
Four species of porcine circoviruses (PCV1-4) have been reported to circulate in Chinese domestic pigs, while the epizootiology of these viruses in free-ranging wild boars in China remains unknown. In this study, tissue and serum samples collected from diseased or apparently healthy wild boars between 2018 and 2020 in 19 regions of China were tested for the prevalence of PCV1-4 infections. Positive rates of PCV1, PCV2, and PCV3 DNA in the tissue samples of Chinese wild boars were 1.6% (4/247), 58.3% (144/247), and 10.9% (27/247) respectively, with none positive for PCV4. Sequence analysis of viral genome showed that the four PCV1 strains distributed in Hunan and Inner Mongolia shared 97.5%-99.6% sequence identity with global distributed reference strains. Comparison of the ORF2 gene sequences showed that 80 PCV2 strains widely distributed in 18 regions shared 79.5%-100% sequence identity with reference strains from domestic pigs and wild boars, and were grouped into PCV2a (7), PCV2b (31) and PCV2d (42). For PCV3, 17 sequenced strains shared 97.2%-100% nucleotide identity at the genomic level and could be divided into PCV3a (3), PCV3b (2) and PCV3c (12) based on the phylogeny of ORF2 gene sequences. Serological data revealed antibody positive rates against PCV1 and PCV2 of 11.4% (19/167) and 53.9% (90/167) respectively. The data obtained in this study improved our understanding about the epidemiological situations of PCVs infection in free-ranging wild boars in China and will be valuable for the prevention and control of diseases caused by PCVs infection.
四种猪圆环病毒（PCV1-4）已被报道在中国国内猪群中流行，而这些病毒在中国野猪中的流行情况尚不清楚。在本研究中，对 2018 年至 2020 年期间来自中国 19 个地区的患病或貌似健康的野猪采集的组织和血清样本进行了 PCV1-4 感染的流行率检测。中国野猪组织样本中 PCV1、PCV2 和 PCV3 DNA 的阳性率分别为 1.6%（4/247）、58.3%（144/247）和 10.9%（27/247），而 PCV4 均为阴性。病毒基因组序列分析表明，分布在湖南和内蒙古的 4 株 PCV1 株与全球分布的参考株的序列同源性为 97.5%-99.6%。ORF2 基因序列比较表明，分布在 18 个地区的 80 株 PCV2 株与来自猪和野猪的参考株的序列同源性为 79.5%-100%，分为 PCV2a（7）、PCV2b（31）和 PCV2d（42）。对于 PCV3，17 个测序株在基因组水平上的核苷酸同源性为 97.2%-100%，根据 ORF2 基因序列的系统发育可分为 PCV3a（3）、PCV3b（2）和 PCV3c（12）。血清学数据显示，针对 PCV1 和 PCV2 的抗体阳性率分别为 11.4%（19/167）和 53.9%（90/167）。本研究获得的数据提高了我们对中国野猪中 PCV 感染流行情况的认识，对预防和控制由 PCV 感染引起的疾病具有重要价值。
