Deheyn D, Mallefet J, Jangoux M
Marine Biology Research Division, Scripps Institution of Oceanography, University of California, La Jolla 92093-0202, USA.
Cell Tissue Res. 2000 Jan;299(1):115-28. doi: 10.1007/s004419900144.
Bioluminescence in the ophiuroid Amphipholis squamata is produced by photocytes located within the spinal ganglia of arm spines. Ganglionic cells were dissociated (pronase digestion) and photocytes separated from other cell types by using a continuous density Percoll gradient. Aliquots from a stock suspension of photocytes in artificial sea water were stimulated to produce light by using KCl or acetylcholine and fixed for ultrastructural observation at different times of the luminous process. Preluminescent, luminescent, and postluminescent photocytes contained various intracytoplasmic structures, such as Golgi, flat and distended rough endoplasmic reticulum cisternae, bundles of fibrils, and up to six types of membrane-bounded vesicles. These structures either co-occurred or succeeded one another during the process of light production, indicating that they were most probably participating in the luminescence reaction. Two types of vesicles, sharing some ultrastructural features, probably represented the microsources of the photocytes. One type occurred almost exclusively in luminescent photocytes, and the other almost exclusively in postluminescent photocytes, suggesting that one may be transformed into the other. The latter type of vesicle contained densely packed fibro-tubular units, giving a characteristic paracrystalline appearance to postluminescent photocytes.
蛇尾纲动物鳞蛇尾（Amphipholis squamata）的生物发光是由位于腕棘脊神经节内的发光细胞产生的。神经节细胞经解离（蛋白酶消化）处理，通过连续密度的Percoll梯度从其他细胞类型中分离出发光细胞。将人工海水中发光细胞储备悬液的等分试样用氯化钾或乙酰胆碱刺激发光，并在发光过程的不同时间固定用于超微结构观察。发光前、发光中和发光后的发光细胞含有各种胞质内结构，如高尔基体、扁平且扩张的粗面内质网池、纤维束以及多达六种类型的膜结合囊泡。这些结构在发光过程中要么同时出现，要么相继出现，表明它们很可能参与了发光反应。两种具有一些超微结构特征的囊泡可能代表了发光细胞的微光源。其中一种几乎只出现在发光的发光细胞中，另一种几乎只出现在发光后的发光细胞中，这表明一种可能会转化为另一种。后一种类型的囊泡含有紧密堆积的纤维管状单元，使发光后的发光细胞呈现出特征性的准晶体外观。
