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[猴血浆小样本中11种类固醇的放射免疫测定法]

[Radioimmunologic method of determining 11 steroids in small samples of monkey blood plasma].

作者信息

Goncharov N P, Chekan S, Antonichev A V, Katsiia G V, Butnev V Iu

出版信息

Vopr Med Khim. 1979 Jan-Feb;25(1):92-7.

PMID:106544
Abstract

A radioimmunological method is described for simultaneous estimation of hestagenes, estrogenes, androgenes and their precursors in small samples of blood plasma obtained from peripheric blood of hamadryads (Papio hamadryas) and macaques (Macaca rhesus). Use of highly specific antisera and of chromatographic isolation of each steroid on columns with celite enabled to estimate the concentration of 11 steroids in 1.1 ml of blood plasma with high accuracy. Standard curve and analysis of its linearity were carried out in the coordinate system logit-log.

摘要

描述了一种放射免疫方法,用于同时测定从阿拉伯狒狒(豚尾狒狒)和猕猴(恒河猴)外周血获取的少量血浆样本中的孕烯醇酮、雌激素、雄激素及其前体。使用高度特异性抗血清以及在硅藻土柱上对每种类固醇进行色谱分离,能够高精度地测定1.1毫升血浆中11种类固醇的浓度。在对数单位-对数坐标系中绘制标准曲线并分析其线性。

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