Goncharov N P, Chekan S, Antonichev A V, Katsiia G V, Butnev V Iu
Vopr Med Khim. 1979 Jan-Feb;25(1):92-7.
A radioimmunological method is described for simultaneous estimation of hestagenes, estrogenes, androgenes and their precursors in small samples of blood plasma obtained from peripheric blood of hamadryads (Papio hamadryas) and macaques (Macaca rhesus). Use of highly specific antisera and of chromatographic isolation of each steroid on columns with celite enabled to estimate the concentration of 11 steroids in 1.1 ml of blood plasma with high accuracy. Standard curve and analysis of its linearity were carried out in the coordinate system logit-log.