Drexler H G
DSMZ-German Collection of Microorganisms & Cell Cultures, Department of Human and Animal Cell Cultures, Braunschweig, Germany.
Leuk Res. 2000 Feb;24(2):109-15. doi: 10.1016/s0145-2126(99)90169-8.
The myelodysplastic syndromes (MDS) are clonal myeloid disorders characterized by bone marrow cell dysplasia and ineffective hematopoiesis leading to peripheral refractory cytopenias. The course of the disease ranges from a chronic status with progressively impaired hematopoiesis to rapid evolution to acute myeloid leukemia (AML). A panel of continuous malignant hematopoietic cell lines has been established from the whole spectrum of MDS variants and also from the different stages of the diseases, namely from the MDS phase or the overt leukemia post-MDS phase. Ten cell lines were derived from the various MDS subtypes; 17 cell lines were established from patients with leukemia (mainly AML) post-MDS. While most cell lines display myelocytic, monocytic or erythroid features, some cell lines carry lymphoid characteristics (precursor B-cell, B-cell, or T-cell), With regard to these lymphoid MDS-derived cell lines, more detailed authentication (prove of derivation from the assumed patient) and verification (prove of the malignant nature of the cell line and derivation from the assumed neoplastic cells) are required to validate the cell lines as true in vitro representatives of MDS and to exclude any cross-contamination with other cells or immortalization of normal bystander cells. On the other hand, lymphoid MDS-derived cell lines may attest to the clonal nature of MDS which may afflict progenitor cells giving rise to lymphoid or myelomonocytoid cells. Many of the MDS-derived cell lines carry cytogenetic and molecular genetic abnormalities typically associated with MDS: gain or loss of all or parts of chromosomes 5, 7, 8 and 20 (-5/5q-, -7/7q-, + 8, 20q-); alterations of oncogenes and tumor suppressor genes (IRF-1, p15, p16, p53, RAS, RB). In summary, the present panel of cell lines provides continuously growing cells and thus unlimited cell material for use as in vitro paradigms covering the whole spectrum of MDS-related hematopoetic malignancies. Properly authenticated and verified MDS-derived cell lines which should be made freely available will represent important research tools for the study of MDS biology.
骨髓增生异常综合征（MDS）是一类克隆性髓系疾病，其特征为骨髓细胞发育异常以及无效造血，进而导致外周血难治性血细胞减少。疾病进程从造血功能逐渐受损的慢性状态到迅速演变为急性髓系白血病（AML）。已从MDS各变异型以及疾病的不同阶段，即MDS期或MDS后的明显白血病期，建立了一组连续的恶性造血细胞系。10个细胞系源自各种MDS亚型；17个细胞系是从MDS后的白血病患者（主要是AML）中建立的。虽然大多数细胞系表现出髓细胞、单核细胞或红系特征，但一些细胞系具有淋巴样特征（前体B细胞、B细胞或T细胞）。对于这些源自MDS的淋巴样细胞系，需要更详细的鉴定（证明源自假定的患者）和验证（证明细胞系的恶性性质以及源自假定的肿瘤细胞），以确认这些细胞系是MDS真正的体外代表，并排除与其他细胞的任何交叉污染或正常旁观者细胞的永生化。另一方面，源自MDS的淋巴样细胞系可能证明MDS的克隆性质，MDS可能影响产生淋巴样或髓单核样细胞的祖细胞。许多源自MDS的细胞系携带通常与MDS相关的细胞遗传学和分子遗传学异常：5号、7号、8号和20号染色体全部或部分的获得或缺失（-5/5q-、-7/7q-、+8、20q-）；癌基因和肿瘤抑制基因（IRF-1、p15、p16、p53、RAS、RB）的改变。总之，目前的细胞系库提供了不断生长的细胞，从而为用作涵盖MDS相关造血恶性肿瘤全谱的体外模型提供了无限的细胞材料。经过适当鉴定和验证且应免费提供的源自MDS的细胞系将成为研究MDS生物学的重要研究工具。
