CENIMAT/I3N, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa and CEMOP/UNINOVA, Campus de Caparica, 2829-516, Caparica, Portugal.
UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516, Caparica, Portugal.
Sci Rep. 2018 Jan 10;8(1):381. doi: 10.1038/s41598-017-18725-9.
Many diseases have their treatment options narrowed and end up being fatal if detected during later stages. As a consequence, point-of-care devices have an increasing importance for routine screening applications in the health sector due to their portability, fast analyses and decreased cost. For that purpose, a multifunctional chip was developed and tested using gold nanoprobes to perform RNA optical detection inside a microfluidic chip without the need of molecular amplification steps. As a proof-of-concept, this device was used for the rapid detection of chronic myeloid leukemia, a hemato-oncological disease that would benefit from early stage diagnostics and screening tests. The chip passively mixed target RNA from samples, gold nanoprobes and saline solution to infer a result from their final colorimetric properties. An optical fiber network was used to evaluate its transmitted spectra inside the chip. Trials provided accurate output results within 3 min, yielding signal-to-noise ratios up to 9 dB. When compared to actual state-of-art screening techniques of chronic myeloid leukemia, these results were, at microscale, at least 10 times faster than the reported detection methods for chronic myeloid leukemia. Concerning point-of-care applications, this work paves the way for other new and more complex versions of optical based genosensors.
许多疾病的治疗选择变得有限，如果在晚期发现，最终可能致命。因此，由于其便携性、快速分析和降低的成本，即时检测设备在医疗保健领域的常规筛查应用中变得越来越重要。为此，开发了一种多功能芯片，并使用金纳米探针进行了测试，以在微流控芯片内进行 RNA 光学检测，而无需分子扩增步骤。作为概念验证，该设备用于快速检测慢性髓性白血病，这是一种血液肿瘤疾病，如果能够进行早期诊断和筛查测试将受益。芯片被动混合来自样本、金纳米探针和生理盐水的目标 RNA，以根据其最终比色特性推断出结果。使用光纤网络评估其在芯片内的传输光谱。试验在 3 分钟内提供了准确的输出结果，产生的信噪比高达 9dB。与慢性髓性白血病的实际最先进的筛选技术相比，这些结果在微尺度上比报道的慢性髓性白血病检测方法至少快 10 倍。就即时护理应用而言，这项工作为其他基于光学的基因传感器的新版本和更复杂版本铺平了道路。
