Trujillo C, Ramírez R, Vélez I D, Berberich C
Programa de Estudio y Control de Enfermedades Tropicales, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
Immunol Lett. 1999 Dec 1;70(3):203-9. doi: 10.1016/s0165-2478(99)00146-7.
The kinetoplastid membrane protein-11 (KMP-11) is a major target of the humoral immune response during Leishmania-infections. The majority of sera from visceral leishmaniasis, mucocutaneous leishmaniasis and even some cutaneous leishmaniasis patients contain detectable IgG antibodies against KMP-11. We also provide evidence that this protein may act as a potent antigen in T. cruzi infections, since most Chagas sera show immunological cross-reactivity. Therefore, KMP-11 cannot be used as a specific diagnostical tool for the serodiagnosis of leishmaniasis in those regions where both, Leishmania and T. cruzi infections overlap geographically. When analyzing the subclass specificity of the antibody response to KMP-11 we observed the following order of reactivity: IgG1 > > IgG3 > IgG2 > IgG4, which is similiar to that seen in crude parasite extract. The mapping of antigenic determinants by using synthetic 20-mer peptides revealed the existence of predominantly conformational epitopes in leishmaniasis, while 50% of sera from Chagas patients reacted with a particular KMP-11 peptide. These results therefore suggest the presence of disease-specific B-cell epitopes.
动基体膜蛋白11（KMP - 11）是利什曼原虫感染期间体液免疫反应的主要靶点。大多数内脏利什曼病、黏膜皮肤利什曼病甚至一些皮肤利什曼病患者的血清中都含有可检测到的抗KMP - 11 IgG抗体。我们还提供证据表明，这种蛋白在克氏锥虫感染中可能作为一种强效抗原，因为大多数恰加斯病血清显示出免疫交叉反应性。因此，在利什曼原虫和克氏锥虫感染在地理上重叠的地区，KMP - 11不能用作利什曼病血清诊断的特异性诊断工具。在分析针对KMP - 11的抗体反应的亚类特异性时，我们观察到以下反应性顺序：IgG1 >> IgG3 > IgG2 > IgG4，这与在粗制寄生虫提取物中观察到的情况相似。通过使用合成的20聚体肽对抗原决定簇进行定位，发现利什曼病中主要存在构象表位，而50%的恰加斯病患者血清与一种特定的KMP - 11肽发生反应。因此，这些结果表明存在疾病特异性的B细胞表位。
