Jensen A T, Gasim S, Ismail A, Gaafar A, Kurtzhals J A, Kemp M, El Hassan A M, Kharazmi A, Theander T G
Centre for Medical Parasitology, Institute for Medical Microbiology and Immunology, University of Copenhagen, Denmark.
Scand J Immunol. 1998 Jul;48(1):103-9. doi: 10.1046/j.1365-3083.1998.00370.x.
Native kinetoplastid membrane protein-11 (KMP-11), purified from crude extracts of Leishmania donovani parasites, activates T cells from individuals who have recovered from visceral leishmaniasis. In this work we used three 38-mer peptides spanning the amino acid sequence of the L. donovani KMP-11 as solid-phase ligands in enzyme-linked immunosorbent assays (ELISAs) and as stimulating antigens in lymphoproliferative assays in order to evaluate humoral and cellular immune responses to well-defined sequences of the protein. Antibody reactivity against the three peptides was measured in plasma from 63 Sudanese visceral leishmaniasis patients (VL) and the percentage of patients with anti-KMP-11 antibodies in ELISA were 37% (KMP-11-1), 30% (KMP-11-2) and 58% (KMP-11-3). The fraction of VL patients with measurable antibody reactivity in one or more of the three ELISAs was 79%. Cross-reactivity to the KMP-11 peptides was detected in plasma from Sudanese patients suffering from Leishmania major infections and in plasma from Sudanese and Danish patients infected with Plasmodium falciparum. In lymphoproliferative assays, 10 of 17 PBMC isolates from donors previously infected with L. donovani showed a response to one or more of the three KMP-11 peptides.
从杜氏利什曼原虫寄生虫粗提物中纯化得到的天然动质体膜蛋白11（KMP - 11），可激活从内脏利什曼病康复者体内分离出的T细胞。在本研究中，我们使用了三种覆盖杜氏利什曼原虫KMP - 11氨基酸序列的38肽，分别作为酶联免疫吸附测定（ELISA）中的固相配体以及淋巴细胞增殖测定中的刺激抗原，以评估针对该蛋白明确序列的体液免疫和细胞免疫反应。在63名苏丹内脏利什曼病患者（VL）的血浆中检测了针对这三种肽的抗体反应性，ELISA中抗KMP - 11抗体阳性患者的比例分别为37%（KMP - 11 - 1）、30%（KMP - 11 - 2）和58%（KMP - 11 - 3）。在三种ELISA中一种或多种检测到可测量抗体反应性的VL患者比例为79%。在患有硕大利什曼原虫感染的苏丹患者血浆以及感染恶性疟原虫的苏丹和丹麦患者血浆中检测到了对KMP - 11肽的交叉反应性。在淋巴细胞增殖测定中，17份来自先前感染杜氏利什曼原虫供体的外周血单核细胞（PBMC）分离物中有10份对三种KMP - 11肽中的一种或多种有反应。
