Agusti R, Couto A S, Alves M J, Colli W, Lederkremer R M
Cihidecar, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Mem Inst Oswaldo Cruz. 2000 Jan-Feb;95(1):97-102. doi: 10.1590/s0074-02762000000100016.
Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites.
克氏锥虫的锥鞭毛体形式用[14C]-乙醇胺和[3H]-棕榈酸进行代谢标记。分析了释放到培养基中的脂质，并与寄生虫成分进行了比较。磷脂酰胆碱和溶血磷脂酰胆碱占总掺入前体的53%。有趣的是，磷脂酰乙醇胺及其溶血衍生物溶血磷脂酰乙醇胺虽然在寄生虫中大量存在，但在释放的物质中却检测不到。与从寄生虫中分离出的总脂肪酸库相比，释放的脂质中高度富集了不饱和脂肪酸C16:1和C18:1。
