Couto A S, Uhrig M L, Agustí R, Befumo M F, Zingales B, Colli W, de Lederkremer R M
Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Biochem Int. 1991 Aug;24(6):991-1002.
Trypomastigotes were metabolically labeled with [3H]-palmitic acid or [3H]-galactose and labeled components were detected in the culture medium. Thin layer chromatography of the shed material showed several lipids in the [3H]-palmitic acid labeled sample while the sugar was mainly incorporated into macromolecules. The material incorporated with the lipidic precursor was fractionated by DEAE-Sephadex (acetate form) and the amount of radioactivity was ten times higher in the acidic lipids than in the neutral lipids. When acidic lipids were further separated by Unisil, 73% of the radioactivity was recovered in the less polar fraction. Different patterns were obtained on comparison of the shed components with the lipids remaining in the parasite.
用[3H]-棕榈酸或[3H]-半乳糖对锥鞭毛体进行代谢标记，并在培养基中检测标记成分。对脱落物质进行薄层色谱分析，结果显示在[3H]-棕榈酸标记的样品中有几种脂质，而糖类主要掺入大分子中。用DEAE-葡聚糖（醋酸盐形式）对掺入脂质前体的物质进行分级分离，酸性脂质中的放射性比中性脂质高10倍。当用硅镁型吸附剂进一步分离酸性脂质时，73%的放射性在极性较小的部分中回收。将脱落成分与寄生虫中残留的脂质进行比较，得到了不同的模式。
