Kolusheva S, Boyer L, Jelinek R
Department of Chemistry, Ben Gurion University of the Negev, Beersheva 84105, Israel.
Nat Biotechnol. 2000 Feb;18(2):225-7. doi: 10.1038/72697.
The increased resistance of various bacteria toward available antibiotic drugs has initiated intensive research efforts into identifying new sources of antimicrobial substances. Short antibiotic peptides (10-30 residues) are prevalent in nature as part of the intrinsic defense mechanisms of most organisms and have been proposed as a blueprint for the design of novel antimicrobial agents. Antimicrobial peptides are generally believed to kill bacteria through membrane permeabilization and extensive pore-formation. Assays providing rapid and easy evaluation of interactions between antimicrobial membrane peptides and lipid bilayers could significantly improve screening for substances with effective antibacterial properties, as well as contribute to the elucidation of structural and functional properties of antimicrobial peptides. Here we describe a colorimetric sensor in which particles composed of phospholipids and polymerized polydiacetylene (PDA) lipids were shown to exhibit striking color changes upon interactions with antimicrobial membrane peptides. The color changes in the system occur because of the structural perturbation of the lipids following their interactions with antimicrobial peptides. The assay was also sensitive to the antibacterial properties of structurally and functionally related peptide analogs.
各种细菌对现有抗生素药物的耐药性增加，引发了对确定新抗菌物质来源的深入研究。短抗菌肽（10 - 30个残基）作为大多数生物体固有防御机制的一部分在自然界中普遍存在，并已被提议作为设计新型抗菌剂的蓝本。抗菌肽通常被认为通过使膜通透化和形成大量孔道来杀死细菌。能够快速且轻松地评估抗菌膜肽与脂质双层之间相互作用的分析方法，可显著改进对具有有效抗菌特性物质的筛选，并有助于阐明抗菌肽的结构和功能特性。在此，我们描述了一种比色传感器，其中由磷脂和聚合聚二乙炔（PDA）脂质组成的颗粒在与抗菌膜肽相互作用时会呈现出显著的颜色变化。系统中的颜色变化是由于脂质与抗菌肽相互作用后其结构受到扰动所致。该分析方法对结构和功能相关的肽类似物的抗菌特性也很敏感。
