Karulin A Y, Hesse M D, Tary-Lehmann M, Lehmann P V
Department of Pathology, Case Western Reserve University, Cleveland, OH 44106, USA.
J Immunol. 2000 Feb 15;164(4):1862-72. doi: 10.4049/jimmunol.164.4.1862.
The patterns of Ag-induced cytokine coexpression in normal, in vivo-primed CD4 memory T cells has remained controversial because the low frequency at which these cells occur has effectively prevented direct ex vivo measurements. We have overcome this limitation by using two-color cytokine enzyme-linked immunospot assays and computer-assisted image analysis. We found CD4 memory cells that simultaneously expressed IL-2, IL-3, IL-4, IL-5, and IFN-gamma to be rare (0-10%). This cytokine segregation was seen in adjuvant-induced type 1, type 2, and mixed immunity to OVA, in Leishmania infection regardless of the Ag dose used or how long after immunization the assay was performed. The data suggest that type 1 and type 2 immunity in vivo is not mediated by classic Th1 or Th2 cells but by single-cytokine-producing memory cells.
在正常的、体内预致敏的CD4记忆T细胞中，抗原诱导的细胞因子共表达模式一直存在争议，因为这些细胞出现的频率较低，有效地阻碍了直接的体外测量。我们通过使用双色细胞因子酶联免疫斑点分析和计算机辅助图像分析克服了这一限制。我们发现同时表达白细胞介素-2、白细胞介素-3、白细胞介素-4、白细胞介素-5和干扰素-γ的CD4记忆细胞很少见（0 - 10%）。在佐剂诱导的针对卵清蛋白的1型、2型和混合免疫中，在利什曼原虫感染中，无论使用的抗原剂量如何，也无论在免疫后多久进行检测，都观察到了这种细胞因子分离现象。数据表明，体内的1型和2型免疫不是由经典的Th1或Th2细胞介导的，而是由产生单一细胞因子的记忆细胞介导的。
