Suppr超能文献

睾丸发育过程中,成纤维细胞生长因子及受体表达在细胞和阶段上的离散定位。

Discrete cell- and stage-specific localisation of fibroblast growth factors and receptor expression during testis development.

作者信息

Cancilla B, Davies A, Ford-Perriss M, Risbridger G P

机构信息

Institute of Reproduction and Development, Monash University, Clayton, Victoria, Australia.

出版信息

J Endocrinol. 2000 Feb;164(2):149-59. doi: 10.1677/joe.0.1640149.

Abstract

Fibroblast growth factors (FGFs) are a family of heparin binding proteins involved in many biological processes. These growth factors act through tyrosine kinase receptors (FGFRs); we have previously used immunohistochemistry to study FGFRs-1-4 in foetal, immature and adult rat testes, and found a discrete cell- and stage-specific localisation. Alternative mRNA splicing of FGFRs-1-3 leads to functional variants (IIIb and IIIc) with distinct ligand binding affinities, therefore we have identified the specific expression of functional FGFR variants and the expression and localisation of FGF ligands in testes from foetal, immature and adult rats. Using reverse transcriptase-polymerase chain reaction (RT-PCR), we found that mRNAs for FGFR-1 IIIb and IIIc, FGFR-2 IIIc, FGFR-3 IIIc and FGFR-4 were expressed in foetal, immature and adult testes. Ligands FGFs-1-5, and -8, which can signal through these receptors, were also expressed in testes at each age. Localisation of the ligands FGFs-1, -3 and -4 to rat testes by immunohistochemistry showed a discrete cell- and stage-specific localisation that altered during testis development. This study has shown that the ligands FGFs-1, -3 and -4 are expressed in the testis and have the capacity to signal through appropriate receptors that are also co-localised or expressed in adjacent cell types in the testis. Collectively, the expression profiles of the seven FGFR variants and FGFs-1-5 and -8 suggest a functional importance in testicular development and spermatogenesis. It is concluded that, future studies on the role of other FGF ligands, in particular FGFs-1-4, are warranted.

摘要

成纤维细胞生长因子(FGFs)是一类参与多种生物学过程的肝素结合蛋白家族。这些生长因子通过酪氨酸激酶受体(FGFRs)发挥作用;我们之前利用免疫组织化学方法研究了胎鼠、未成熟大鼠和成年大鼠睾丸中的FGFRs-1-4,发现了离散的细胞和阶段特异性定位。FGFRs-1-3的可变mRNA剪接导致具有不同配体结合亲和力的功能变体(IIIb和IIIc),因此我们确定了功能性FGFR变体的特异性表达以及FGF配体在胎鼠、未成熟大鼠和成年大鼠睾丸中的表达和定位。使用逆转录聚合酶链反应(RT-PCR),我们发现FGFR-1 IIIb和IIIc、FGFR-2 IIIc、FGFR-3 IIIc和FGFR-4的mRNA在胎鼠、未成熟大鼠和成年大鼠睾丸中均有表达。可通过这些受体发出信号的配体FGFs-1-5和-8在各年龄段的睾丸中也有表达。通过免疫组织化学将配体FGFs-1、-3和-4定位到大鼠睾丸,结果显示其具有离散的细胞和阶段特异性定位,且在睾丸发育过程中发生变化。这项研究表明,配体FGFs-1、-3和-4在睾丸中表达,并有能力通过同样在睾丸中相邻细胞类型中共定位或表达的合适受体发出信号。总体而言,七种FGFR变体以及FGFs-1-5和-8的表达谱表明它们在睾丸发育和精子发生中具有功能重要性。得出的结论是,有必要对其他FGF配体,特别是FGFs-1-4的作用进行进一步研究。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验