Chen Lanlan, Li Xiaoheng, Wang Yiyan, Song Tiantian, Li Huitao, Xie Lubin, Li Linchao, Chen Xianwu, Ma Leikai, Chen Yong, Lv Yao, Li Xingwang, Ge Ren-Shan
Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
Department of Anesthesiology, Taizhou People's Hospital, The Fifth Hospital Affiliated Nantong University, Taizhou, China.
Front Endocrinol (Lausanne). 2019 Mar 8;10:118. doi: 10.3389/fendo.2019.00118. eCollection 2019.
Fibroblast growth factor 1 (FGF1) is reported to be expressed in the testis. How FGF1 affects stem Leydig cell development remains unclear. Here, we report the effects of FGF1 on rat stem Leydig cell development in an ethane dimethane sulfonate (EDS)-treated model. FGF1 (100 ng/testis) significantly increased serum testosterone level, increased PCNA-positive Leydig cell percentage and Leydig cell number, but down-regulated the expression of , and in Leydig cells , after its daily intratesticular injection from post-EDS day 14 for 14 days. Primary culture of the seminiferous tubules showed that FGF1 stimulated EdU incorporation to stem Leydig cells but blocked the differentiation into the Leydig cell lineage, possibly via FGFR1-mediated mechanism. In conclusion, FGF1 promotes stem Leydig cell proliferation but blocks its differentiation.
据报道，成纤维细胞生长因子1（FGF1）在睾丸中表达。FGF1如何影响睾丸间质干细胞的发育尚不清楚。在此，我们报告了FGF1对乙烷二甲磺酸盐（EDS）处理模型中大鼠睾丸间质干细胞发育的影响。从EDS处理后第14天开始，每天进行睾丸内注射FGF1（100 ng/睾丸），持续14天，结果显示FGF1显著提高了血清睾酮水平，增加了PCNA阳性睾丸间质细胞百分比和睾丸间质细胞数量，但下调了睾丸间质细胞中、和的表达。曲细精管的原代培养表明，FGF1刺激了睾丸间质干细胞的EdU掺入，但可能通过FGFR1介导的机制阻断了其向睾丸间质细胞谱系的分化。总之，FGF1促进睾丸间质干细胞增殖，但阻断其分化。
