噬菌体fl DNA的合成起源与方向
Origin and direction of synthesis of bacteriophage fl DNA.
作者信息
Horiuchi K, Zinder N D
出版信息
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2341-5. doi: 10.1073/pnas.73.7.2341.
Abstract
The origin and direction of synthesis in vivo of the viral and complementary strands of f1 DNA were studied by measuring the distribution of radioactivity along the genome after a short pulse of [3H]thymidine. The results indicate that the origins of replication of viral and complementary strands are located close to one another, probably both within a restriction fragment (HaeIII-G) which is about 120 bases long. Replication of both viral and complementary strands proceeds in the 5' leads to 3' overall direction. Thus, the viral strand is elongated in the counterclockwise and the complementary strand in the clockwise direction on the standard genetic map. A model is proposed in which only two mechanisms are invoked to generate all f1 DNA species: (1) the conversion of single-stranded viral DNA into double-stranded molecules and (2) the synthesis of viral single strands from double strands.
摘要
通过在短时间脉冲加入[³H]胸腺嘧啶后测量放射性沿基因组的分布，研究了f1 DNA病毒链和互补链在体内的合成起始点和方向。结果表明，病毒链和互补链的复制起始点彼此靠近，可能都在一个约120个碱基长的限制片段（HaeIII - G）内。病毒链和互补链的复制总体上都沿5'到3'方向进行。因此，在标准遗传图谱上，病毒链沿逆时针方向延长，互补链沿顺时针方向延长。提出了一个模型，其中仅调用两种机制来产生所有f1 DNA种类：（1）单链病毒DNA转化为双链分子；（2）从双链合成病毒单链。
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