Hourcade D, Dressler D
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1652-6. doi: 10.1073/pnas.75.4.1652.
The first step in the replication cycle of the single-stranded DNA phages is the conversion of the infecting positive strand circle to a duplex ring. This event involves the de novo initiation of a negative strand, using the infecting positive strand cycle as a template. The synthesis of the negative strand is in many respects analogous to the formation of a fragment during cellular DNA replication. In this paper we describe the initiation of the negative strand of bacteriophage G4. The data establish that, in vivo, the synthesis of the G4 negative strand is initiated at a specific site, which we have mapped on the 5400-base viral chromosome.
单链DNA噬菌体复制周期的第一步是将感染性正链环转化为双链环。这一过程涉及以感染性正链环为模板从头起始合成负链。负链的合成在许多方面类似于细胞DNA复制过程中片段的形成。在本文中，我们描述了噬菌体G4负链的起始合成。数据表明，在体内，G4负链的合成起始于一个特定位点，我们已将该位点定位在5400个碱基的病毒染色体上。
